低氧诱导因子1α在小鼠下颌第一磨牙萌出过程中的表达及意义

Expression of hypoxia inducible factor-1alpha during mouse mandibular first molar eruption and its significance

目的:研究小鼠下颌第一磨牙萌出过程中低氧诱导因子1α(hypoxia inducible factor-1alpha,HIF-1α)的表达,并探讨其表达与牙萌出及破骨细胞活性之间的关系。方法:分离出生后1.5~14.5 d小鼠的下颌骨,连续切片,对每组切片进行苏木精-伊红(H-E)染色、抗酒石酸酸性磷酸酶染色以及免疫组织化学染色。采用SPSS 5.0软件包进行统计学分析。结果:小鼠下颌第一磨牙萌出过程中,其冠方牙槽骨厚度逐渐减小,破骨细胞数量逐渐减少,HIF-1α在萌出过程中的表达逐渐减少。结论:小鼠下颌第一磨牙萌出过程中,HIF-1α表达越少,破骨细胞数量也越少。HIF-1α可能通过影响破骨细胞活性而参与小鼠下颌第一磨牙的萌出。

PURPOSE:To investigate the expression of hypoxia inducible factor-1 alpha(HIF-1α)during the eruption of mouse lower first molar,and to explore the correlation between its expression and the activity of osteoclasts.METHODS:Mouse mandibles were dissected from mice at day 1.5 to 14.5 after birth.They were then serially sectioned and stained respectively using hemotoxylin,tartrate-resistant acid phosphatase(TRAP)staining,and immunohistochemical staining.SPSS 25.0 software package was used for data analysis.RESULTS:During the eruption of mouse lower first molar,the thickness of the crown alveolar bone gradually decreased,so did the number of osteoclasts and the expression of HIF-1α.CONCLUSIONS:During the eruption of mouse lower first molar,the less expression of HIF-1α,the fewer osteoclasts there were.HIF-1αmay be involved in the eruption of mouse lower first molar by regulating the activity of osteoclasts.