低密度脂蛋白在钛金属表面的吸附行为分析

Adsorption behavior of low density lipoprotein on titanium surface

目的:初步研究低密度脂蛋白(low density lipoprotein,LDL)在钛金属表面的吸附行为。方法:采用不同浓度(0、1、2 mg/mL)的LDL浸泡纯钛试件,利用低密度脂蛋白浓度测试盒测量LDL浸泡前、后的浓度变化,采用扫描电镜(scanning electron microscopy,SEM)、X射线光电子能谱分析(X-ray photoelectron spectroscopy,XPS)观察分析LDL在钛表面的吸附及其对钛表面元素组成的影响,并检测LDL吸附前、后的钛表面接触角。采用SPSS 22.0软件包对数据进行统计学分析。结果:随着时间延长,LDL在钛表面的吸附逐渐增多。XPS分析结果显示,LDL吸附于钛表面后,钛表面的钛和氧元素含量降低,且钛表面的亲水性减弱。结论:LDL能够吸附于钛金属表面,在一定程度上改变钛表面的理化特性。

PURPOSE:The purpose of this study was to investigate the adsorption of low density lipoprotein(LDL)on titanium surface.METHODS:Pure titanium specimens were soaked with different concentrations of LDL(0,1,2 mg/mL).Low density lipoprotein essay was used to analyze the change of LDL adsorption on titanium surface with time going on.Scanning electron microscope(SEM)and X-ray photoelectron spectroscopy(XPS)were used to observe and analyze the adsorption of LDL on titanium surface and its effect on element composition of titanium surface.Contact angle of titanium surface was detected before and after LDL adsorption.SPSS 22.0 software package was used for statistical analysis of the data.RESULTS:The adsorption amount of LDL on titanium surface gradually increased as time went on.XPS analysis results showed that LDL was adsorbed on the titanium surface,the content of titanium and oxygen elements seemed to decrease on the titanium surface,and the hydrophilicity of the titanium surface declined.CONCLUSIONS:LDL can adsorb on titanium surface,which can change the physical and chemical properties of titanium surface to a certain extent.