DZIP1通过影响Wnt/β-catenin信号通路促进口腔鳞状细胞癌增殖、迁移和侵袭

DZIP1 promotes proliferation,migration,and invasion of oral squamous cell carcinoma by affecting the Wnt/β-catenin signaling pathway

目的探究DZIP1通过Wnt/β-catenin信号通路对口腔鳞状细胞癌增殖、迁移和侵袭的影响。方法实时荧光定量PCR和Western blot检测DZIP1和Wnt/β-catenin信号通路相关基因mRNA表达和蛋白表达,CCK-8检测细胞活力,克隆形成实验检测口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)细胞增殖能力,Transwell实验检测细胞迁移和侵袭能力。结果与人口腔角质形成细胞相比,人OSCC细胞系TSCCA,Tca8113和SCC25中DZIP1的mRNA表达和蛋白表达均显著升高;与阴性对照组(negative control,NC)相比,敲低DZIP1显著降低SCC25细胞Wnt/β-catenin信号通路相关基因的mRNA表达和蛋白表达(P<0.05),显著降低细胞活力(P<0.05),显著抑制细胞增殖(P<0.05),显著降低细胞迁移和侵袭能力(P<0.05),LiCl显著升高SCC25细胞Wnt/β-catenin信号通路相关基因的mRNA表达和蛋白表达(P<0.05),显著升高细胞活力(P<0.05),显著促进细胞增殖(P<0.05),显著升高细胞迁移和侵袭能力(P<0.05);与si-DZIP1组相比,si-DZIP1+LiCl显著升高SCC25细胞Wnt/β-catenin信号通路相关基因的mRNA表达和蛋白表达(P<0.05),显著升高细胞活力(P<0.05),显著促进细胞增殖(P<0.05),显著升高细胞迁移和侵袭能力(P<0.05)。结论DZIP1通过影响Wnt/β-catenin信号通路促进口腔鳞状细胞癌增殖、迁移和侵袭。

Objective To investigate the effect of DZIP1 on the proliferation,migration and invasion of oral squamous cell carcinoma through the Wnt/β-catenin signaling pathway.Methods Real-time quantitative PCR and Western blot were used to detect the mRNA and protein expression of genes related to DZIP1 and Wnt/β-catenin signaling pathway.Cell viability was detected by CCK-8.Oral squamous cell carcinoma(OSCC)was detected by clone formation to assay cell proliferation ability.Transwell assay was used to detect cell migration and invasion.Results Compared with human oral keratinocytes(HOK),mRNA expression and protein expression of DZIP1 in human OSCC cell lines TSCCA,Tca8113 and SCC25 were significantly increased.Compared with the negative control(NC)group,knocking down DZIP1 significantly reduced the mRNA and protein expression of Wnt/β-catenin signaling pathway-related genes in SCC25 cells(P<0.05),and reduced cell viability(P<0.05),migration and invasion ability,inhibited cell proliferation(P<0.05).LiCl significantly increased the mRNA and protein expression of genes related to Wnt/β-catenin signaling pathway in SCC25 cells(P<0.05),increased cell viability(P<0.05),promoted cell proliferation(P<0.05),migration and invasion ability(P<0.05).Compared with the si-DZIP1 group,si-DZIP1+LiCl significantly increased the mRNA and protein expression of genes related to the Wnt/β-catenin signaling pathway in SCC25 cells(P<0.05),and increased cell viability(P<0.05),promoted cell proliferation(P<0.05),migration and invasion capacity(P<0.05).Conclusion DZIP1 promotes the proliferation,migration and invasion of oral squamous cell carcinoma by affecting the Wnt/β-catenin signaling pathway.