Livin表达与口腔鳞状细胞癌上皮间质转化的关系及其临床意义

Relationship between Livin expression and epithelial-mesenchymal transformation in oral squamous cell carcinoma and its clinical significance

目的探讨Livin蛋白和上皮间质转化(EMT)相关蛋白Vimentin、E-cadherin在口腔鳞状细胞癌(OSCC)组织中的表达,探讨三者表达的相关性及其临床意义。方法应用免疫组化EliVision法检测56例OSCC组织及56例癌旁组织中Livin、Vimentin、E-cadherin的表达,并分析三者表达的相关性及其与OSCC患者临床病理特征的关系。进一步应用Western blotting检测10例新鲜OSCC组织中Livin、Vimentin、E-cadherin蛋白的表达水平。结果Livin在OSCC组织中的阳性率为67.9%,明显高于癌旁组织中Livin的阳性率(16.1%),且Livin表达与OSCC的淋巴结转移及TNM分期相关。E-cadherin、Vimentin在OSCC组织中的阳性率分别为44.6%、50.0%,Livin与E-cadherin表达呈负相关,与Vimentin表达呈正相关。Western blotting检测结果显示Livin高表达的OSCC组织中,E-cadherin呈低表达,Vimentin呈高表达。结论Livin表达可促进OSCC侵袭转移,这种促进作用可能是通过表达Livin从而激活上皮间质转化(EMT)发生实现的。

Objective To investigate the expression of Livin protein and epithelial-mesenchymal transformation(EMT)-related proteins Vimentin and E-cadherin in oral squamous cell carcinoma(OSCC)tissues,and to explore the correlation and clinical significance of the three protein expressions.Methods EliVision method of immunohistochemical was used to examine the expression of Livin,Vimentin and E-cadherin in 56 cases of OSCC and 56 cases of paracancerous tissues.The correlation between the expression of Livin,Vimentin and E-cadherin and the clinicopathological features of OSCC patients was analyzed.Furthermore,western blot was used to examine the expression of Livin,Vimentin and E-cadherin in 10 fresh OSCC tissues.Results The positive rate of Livin in OSCC tissues was 67.9%,which was significantly higher than that in paracancerous tissues(16.1%),and the expression of Livin was related to lymph node metastasis and TNM stage of OSCC.The positive rates of E-cadherin and Vimentin in OSCC tissues were 44.6%and 50%,respectively.Livin was negatively correlated with the expression of E-cadherin and positively correlated with the expression of Vimentin.The results of western blot showed that in the OSCC tissues with high expression of Livin,the expression of E-cadherin was low and Vimentin was high.Conclusion The expression of Livin can promote the invasion and metastasis of OSCC,which may be achieved by activating the occurrence of EMT by expressing Livin.