AGXT2基因多态性与草酸钙肾结石复发的相关性分析

Correlation between AGXT2 gene polymorphism and recurrence of calcium oxalate nephrolithiasis

目的:分析丙氨酸乙醛酸转氨酶2(AGXT2)基因多态性与草酸钙肾结石复发的相关性。方法:选择2017年1月~2019年12月本院随访的草酸钙肾结石复发患者136例为研究对象(复发组),另匹配同期初发草酸钙肾结石患者140例为对照(初发组)。提取血液基因组DNA,采用DNA PCR测序技术检测AGXT2基因rs37369位点基因多态性,采用Logistic回归分析AGXT2 rs37369位点多态性与草酸钙肾结石患者复发的关系。结果:复发组与初发组草酸钙肾结石患者性别、年龄、BMI、高血压、糖尿病比例、AG基因型频率比较差异均无统计学意义(P>0.05)。与初发组比较,复发组血清SCr、BUN水平、AGXT2基因rs37369位点A等位基因、AA基因型频率增加(P<0.05),GFR、AGXT2基因rs37369位点G等位基因、GG基因型频率显著降低(P<0.05)。与携带GG基因型患者比较,携带AA基因型患者SCr、BUN水平显著升高(P<0.05),GFP显著降低(P<0.05)。Logistic回归分析结果显示,携带AA基因型是草酸钙肾结石复发的独立危险因素(P<0.05)。结论:AGXT2基因rs37369位点多态性可能与草酸钙肾结石复发有一定相关性,携带AA基因型是其独立危险因素。

Objective:To analyze the correlation between the polymorphism of alanine glyoxylate transaminase 2(AGXT2)gene and the recurrence of calcium oxalate nephrolithiasis.Method:From January 2017 to December 2019,136 patients with recurrence of calcium oxalate nephrolithiasis followed up in our hospital were selected as the study objects(recurrence group),while 140 patients with primary calcium oxalate nephrolithiasis in the same period were matched as the control(primary group).The genomic DNA of blood was extracted,the rs37369 polymorphism of AGXT2 gene was detected by DNA PCR sequencing technology,and the relationship between AGXT2 rs37369 polymorphism and recurrence of calcium oxalate nephrolithiasis was analyzed by Logistic regression.Result:There was no significant difference in sex,age,BMI,hypertension,diabetes ratio or AG genotype frequency between recurrence group and primary group(P>0.05).Compared with the primary group,the level of serum SCr,BUN,the frequencies of A allele and AA genotype at rs37369 of AGXT2 gene in the recurrence group increased(P<0.05),but the GFP,the frequencies of G allele and GG genotype at rs37369 of AGXT2 gene significantly decreased(P<0.05).Compared with the patients with GG genotype,the levels of SCr and BUN in patients with AA genotype were significantly higher(P<0.05),but GFP was significantly lower(P<0.05).Logistic regression analysis showed that AA genotype was an independent risk factor for the recurrence of calcium oxalate nephrolithiasis(P<0.05).Conclusion:The rs37369 polymorphism of AGXT2 gene may be related to the recurrence of calcium oxalate nephrolithiasis,and AA genotype is an independent risk factor.