肠道菌群在血清tIgE水平升高的变应性鼻炎儿童发病中的作用

Role of gut microbiota in children with allergic rhinitis with high serum total IgE level

目的:探究肠道菌群在血清tIgE水平升高的变应性鼻炎儿童发病中的作用。方法:纳入上海交通大学医学院附属上海儿童医学中心收治的5~6级重度户尘螨过敏的变应性鼻炎患儿17例,收集所有受试者一般临床资料,采集外周血检测其血清tIgE、sIgE水平。收集发作期、控制期粪便样本进行细菌DNA提取,并行16S rDNA高通量测序。采用R软件、Mother软件以及LEfSe软件进行肠道菌群多样性、相对丰度及差异物种分析。结果:共收集粪便样本23份,其中发作期13份(发作组),控制期10份(对照组)。发作期样本中血清tIgE阳性6例(阳性组),阴性7例(阴性组)。与控制期相比,变应性鼻炎患儿发作期肠道菌群的Alpha多样性、Weighted Unifrac的Beta多样性无显著差异(P>0.05)。臭杆菌科臭杆菌属相对丰度在发作期患儿中显著升高(LDA绝对值>2)。血清tIgE阳性患儿卟啉单胞菌科相对丰度下降(LDA绝对值>2),显著低于阴性患儿,而脱硫代弧菌科Pyramidobacter属相对丰度增加。结论:儿童变应性鼻炎发作期和控制期具有不同的肠道菌群特征,肠道菌群与儿童变应性鼻炎血清tIgE水平升高有关,特定微生物改变在患儿发病中发挥潜在作用。

Objective:To study the role of gut microbiota in children with allergic rhinitis with high serum total IgE level.Method:A total of 17 cases of children in Shanghai Children’s Medical Center of Shanghai Jiao Tong University School of Medicine,who suffered from perennial allergic rhinitis of grade 5-6,were enrolled in this study.Baseline information were collected from all participants.Peripheral blood was collected to test the level of serum total IgE and specific IgE.Fecal samples were collected for bacterial DNA extraction and sequenced by 16 S rDNA high-throughput sequencing.R,Mother and LEfSe softwares were used for diversity analysis,relative abundance calculation and differential species detection.Result:Twenty-three fecal samples were collected in total,including thirteen in attack period(attack group)and ten in control period(control group).In the attack group,six cases were serum total IgE positive(positive group)and seven were negative(negative group).Compared with the control group,there was no significant difference in either Alpha diversity or Beta diversity of Weighted Unifrac in the attack group(P>0.05).The relative abundance of odoribacteraceae and odoribacter were significantly increased in the attack group(LDA score>2).The relative abundance of Porphyromonadaceae in positive group were significantly lower than that in negative group,while family Dethiosulfovibrionaceae genus Pyramidobacter was definitely higher on the contrast(LDA score>2).Conclusion:Children with allergic rhinitis have different characteristics of intestinal flora during the attack and control period.Gut microbiota is associated with high serum total IgE level in children with allergic rhinitis.Specific microbial alterations play a potential role in disease pathophysiology.