欧洲葡萄‘粉红亚都蜜’NAC基因DRL1负向调节植物抗旱性

DRL1,a NAC Gene from Vitis vinifera‘Yatomo Rose’,Negatively Regulates the Drought Tolerance

以欧洲葡萄‘粉红亚都蜜’(Vitis vinifera‘Yatomo Rose’)为材料,利用荧光定量PCR技术和转基因技术研究葡萄NAC转录因子DRL1基因对逆境的响应。欧洲葡萄‘粉红亚都蜜’在激素和逆境胁迫下,DRL1表达呈下降趋势,其中以ABA和干旱胁迫处理最为显著。在ABA处理下DRL1转基因烟草株系种子萌发率和根长均高于野生型。干旱处理下,转基因植株对干旱的耐受性降低,同时胁迫相关基因NtLEA5、NtP5CR1、NtPSCS1、NtERD10C和NtDREB3的表达水平比野生型显著下降。此外,DRL1转基因烟草茎中柱发育受到抑制,尤其是导管横切面积仅为野生型的58%。以上结果表明,DRL1基因可能作为1个负向调节子参与植物的干旱胁迫。

The function of NAC transcription factor DRL1,isolated from Vitis vinifera‘Yatomo Rose’,was characterized using the fluorescence quantitative PCR and transgenic technology.Under the hormone and stress treatments,the expression of DRL1 decreased with the strongest reduction in ABA and drought treatments.Exposed to ABA,DRL1 transgenic plants reduced the germination rate and root length.Treated with drought stress,DRL1 enhanced the sensitivity to drought tolerance and the stress related genes,such as NtLEA5,NtP5 CR1,NtPSCS1,NtERD10 C and NtDREB3,were down-regulated in transgenic plants.Moreover,DRL1 restrained the development of stele,and the vessel area was only 58%of wild tobacco.According to these results,DRL1 might negatively regulate drought stress.