常规IVF完全受精失败患者的ZP3基因突变检测分析

Detection and analysis of ZP3 gene mutation in patients with complete fertilization failure of conventional IVF

目的通过Sanger测序,检测ZP3基因突变在常规IVF受精正常组和完全受精失败组的出现频率情况,以寻求突变对受精的影响。方法选取接受常规IVF治疗的患者90例,按受精率分为受精正常组(对照组 n=64)和完全受精失败组(试验组 n=26),收集女性患者血液样本并提取DNA;根据ZP3设计引物进行扩增,利用Sanger测序,观察两组ZP3基因突变情况。结果共发现8种基因突变,突变在对照组和实验组中出现频率均没有显著性差异(P>0.05)。结论 8种ZP3基因突变可能和受精无关系,尚需大样本和多因素研究。

Objective:The frequency of ZP3 gene mutation in normal IVF fertilization group and complete fertilization failure group was detected by Sanger sequencing in order to find out the effect of mutation on fertilization.Methods:90 patients receiving conventional IVF treatment were divided into normal fertilization group(control group n=64)and complete fertilization failure group(experimental group n=26).Blood samples of female patients were collected and DNA was extracted.Primers were designed according to ZP3 for amplification and Sanger sequencing were used to observe the mutation of ZP3 gene in two groups,and the mutation of ZP3 gene in two groups was observed.Results:A total of 8 ZP3 gene mutations were found.There was no significant difference in the frequency of mutations between the control group and the experimental group.Conclusion:8 ZP3 gene mutations may not be related to fertilization,and large samples and multivariate studies are needed.