Cx26在D-半乳糖胺诱导的急性肝损伤中的表达

Expression of Cx26 in Acute Liver Injury Induced by D-galactosamine

目的:研究连接蛋白Cx2.6在D-半乳糖胺诱导的大鼠急性肝损伤中的表达及意义。方法:建立D-半乳糖胺诱导大鼠急性肝损伤模型,用联苯双酯干预作为阳性对照组,检测肝体比指数、血清中天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)的含量;采用显微镜观察大鼠肝脏的病理改变;采用蛋白印迹法(WB)对Cx26蛋白进行定量分析;用实时荧光定量聚合酶链反应(Real-time qPCR)检测Cx26的mRNA表达情况。结果:D-半乳糖胺引起大鼠肝脏急性损伤,可见细胞坏死,引发炎症反应;AST、ALT含量明显升高(P<0.05);Cx26的蛋白含量显著增加(P<0.05),而其mRNA的含量明显减少(P<0.01)。给予联苯双酯干预后,未改变D-半乳糖胺所致肝损伤时Cx26蛋白及mRNA的异常表达。结论:D-半乳糖胺可致大鼠肝脏中Cx26表达异常,这可能是其致病机理之一,可为肝损伤的治疗提供一个潜在的靶点。

Objective:To study the effect of Connexin(Cx)26 on acute liver injury induced by D-galactosamine in rats.Methods:To establish the model of acute liver injury induced by D-galactosamine,bifendatatum was used as positive drug.The liver-body ratio,serum aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels were measured.Pathological changes were observed by microscope.Cx26 protein was quantitatively analyzed by Western Blot(WB).The mRNA expression of Cx26 was detected by real-time quantitative polymerase chain reaction(Real-time qPCR).Results:D-galactosamine caused acute liver injury,cell necrosis was observed and inflammatory reaction was induced.The content of AST and ALT was significantly increased(P<0.05).The protein content of Cx26 in the liver increased significantly(P<0.05),while the mRNA content decreased significantly(P<0.01).Intervention with bifendatatum did not reverse the damage caused by D-galactosamine and the abnormal expression of Cx26.Conclusion:D-galactosamine can cause abnormal increase of Cx26 protein expression in rat liver,which may be one of the pathogenic mechanisms,and also provide a potential target for the treatment of liver injury.