DMEM和F-12K对肝癌细胞系HepG-2的影响

背景:HepG-2人肝肿瘤细胞株在遗传毒理学、细胞毒性等方向有广泛的研究。DMEM和F-12K是两种非常常见的培养基,然而在不同培养基中关于肝癌细胞系HepG-2生长状况的研究和文献资料十分罕见。目的:对比10%胎牛血清1%双抗的DMEM培养基和10%胎牛血清1%双抗的F-12K培养基培养的HepG-2细胞的状态。方法:分别用高糖DMEM和F-12K培养基培养HepG-2细胞,在细胞生长的阶段中用倒置显微镜观察并记录细胞生长状态。结果与结论:在DMEM中培养状态良好的HepG-2细胞不能或无法以正常的形态在F-12K培养基中生长,因此与HepG-2细胞有关的细胞培养需要用DMEM培养基培养。

Background:HepG-2 human liver tumor cell line has been widely studied in genetic toxicology and cytotoxicity.DMEM and F-12K are two very common media.However,the research and literature on the growth of HepG-2 cells in different media are very rare.Objective:In order to compare the status of HepG-2 cells cultured in 10%FBS 1%DMEM media and 10%FBS 1%double antibody F-12K media.Methods:HepG-2 cells were cultured in high glucose DMEM and F-12K media respectively.The growth state of HepG-2 cells was observed and recorded by inverted microscope during cell growth.Results and Conclusion:Those HepG-2 cells that grow well in DMEM could not or could not grow in F-12K media in normal form.Therefore,cell culture related to HepG-2 cells should be cultured in DMEM media.