LncRNA OIP5-AS1靶向miR-511-3p抑制肺癌细胞的增殖、迁移和侵袭的影响

LncRNA OIP5⁃AS1 targeting miR⁃511⁃3p inhibits the proliferation,migration and invasion of lung cancer cells

目的研究LncRNA OIP5-AS1对肺癌细胞的增殖、迁移和侵袭的影响以及作用机制。方法采用qRT-PCR法检测OIP5-AS1和miR-511-3p的表达量;采用MTT法检测细胞增殖;采用Transwell小室实验检测细胞迁移和侵袭;蛋白免疫印迹法检测CyclinD1、MMP-2蛋白表达量;双荧光素酶报告实验检测OIP5-AS1和miR-511-3p的靶向关系。结果与正常细胞肺上皮细胞BEAS-2B相比,肺癌细胞A549、NCI-H23和NCI-H2170中OIP5-AS1表达显著增加,miR-511-3表达显著降低,差异有统计学意义(P<0.05);敲低OIP5-AS1或者过表达miR-511-3p显著抑制了肺癌细胞A549的增殖、迁移和侵袭;OIP5-AS1可以靶向负调控miR-511-3p的表达,抑制miR-511-3p可以部分回复敲低OIP5-AS1对肺癌细胞A549的增殖、迁移和侵袭的影响(P<0.05)。结论 LncRNA OIP5-AS1通过靶向调控miR-511-3p的表达抑制肺癌细胞的增殖、迁移和侵袭。

Objective To study the effect of LncRNA OIP5⁃AS1 on the proliferation,migration and invasion of lung cancer cells,and its mechanism.Methods qRT⁃PCR method was used to detect the expression of OIP5⁃AS1 and miR⁃511⁃3p;MTT method was used to detect cell proliferation;Transwell assay experiment was used to detect cell migration and invasion;Western blotting method were used to detect CyclinD1 and MMP⁃2 protein expression;and the dual luciferase reporter experiment detects the targeting relationship between OIP5⁃AS1 and miR⁃511⁃3p.Results Compared with normal lung epithelial BEAS⁃2B cells,the expression of OIP5⁃AS1 in lung cancer cells A549,NCI⁃H23 and NCI⁃H2170 was significantly increased,and the expression of miR⁃511⁃3p was significantly decreased;knockdown OIP5⁃AS1 or overexpress miR⁃511⁃3p significantly inhibits the proliferation,migration(72.42±7.73 and 68.52±5.63)and invasion of lung cancer cell A549;OIP5⁃AS1 can target and negatively regulate the expression of miR⁃511⁃3p,and inhibit miR⁃511⁃3p to reverse the effects of partial knockdown OIP5⁃AS1 on the proliferation,migration and invasion of lung cancer cells A549(P<0.05).Conclusion LncRNA OIP5⁃AS1 inhibits the proliferation,migration and invasion of lung cancer cells through targeted regulation of the expression of miR⁃511⁃3p.