基于生物信息学分析F2R基因在胃癌中表达与免疫浸润的临床意义

Clinical Significance of F2R Gene Expression and Immune Infiltration in Gastric Cancer Based on Bioinformatics Analysis

目的探究凝血因子II(凝血酶)受体(F2R)的在胃癌中的表达与免疫细胞浸润情况,探索F2R在胃癌中预后价值及其生物学功能。方法从TCGA数据库中下载胃癌中F2R基因表达谱数据及患者的临床病理信息,使用R4.0.2软件提取F2R在胃癌组及正常对照组的表达量数据,比较两组间F2R的表达差异。利用Kaplan-Meier法分析F2R表达与患者预后的相关性,并利用GEPIA对此进行验证。使用Cox回归分析F2R表达与胃癌患者临床预后相关性。利用GSEA富集分析预测F2R在胃癌中参与的信号通路。使用TIMER2.0分析了F2R表达与免疫浸润之间的关系。结果 F2R在胃癌样本中表达显著上调(P<0.05);生存分析显示F2R高表达组的总体生存率显著高于低表达组,差异均有统计学意义(P<0.05);F2R的表达也与肿瘤组织学分级、临床分期和及肿瘤状态相关,是胃癌的独立预后因素(HR=1.303,95%CI:1.076~1.578,P<0.05)。富集分析结果显示,F2R基因主要富集在MTOR信号通路、MAPK信号通路和癌症通路等。免疫浸润分析结果显示F2R表达与CD4+T细胞、CD8+T细胞、巨噬细胞相关(Rho>0.5,P<0.05)。结论 F2R在胃癌中表达显著上调,可作为胃癌患者的独立预后因子,且与免疫浸润有关。

Objective To explore the expression of F2R in gastric cancer and immune cell infiltra⁃tion and the prognostic value of F2R in gastric cancer and its biological function.Methods F2R expression profile data and clinicopathological information of gastric cancer patients were downloaded from TCGA data⁃base.R4.0.2 software was used to extract the expression data of F2R in the gastric cancer group and the normal control group.The expression differences between the two groups were analyzed.Kaplan⁃Meier method was used to perform the survival analysis,GEPIA were used to verify the prognostic value.Cox regression models were adopted to analyze the clinicophathologic features.GSEA enrichment analysis was used to predict the sig⁃naling pathways that F2R participates in gastric cancer.TIMER2.0 was used to analyze the relationship be⁃tween F2R expression and immune infiltration.Results The expression level of F2R in gastric cancer was sig⁃nificantly up⁃regulated(P<0.05).The overall survival rate of F2R in the high expression group was lower than that of the low expression group.The expression level of F2R was significantly related to tumor histological grade(P<0.05),clinical stage(P<0.05)and tumor status(P<0.05).F2R was an independent risk factor for the prognostic of gastric cancer(HR=1.303,95%CI:1.076~1.578,P<0.05).GSEA enrichment results indicated that F2R was enriched in the MTOR signaling pathway,MAPK signaling pathway and cancer pathway.Im⁃mune infiltration analysis showed that F2R expression was correlated with CD4+T cells,CD8+T cells and mac⁃rophages(Rho>0.5,P<0.05).Conclusion F2R is significantly up⁃regulated in gastric cancer,which can be used as an independent prognostic factor in patients with gastric cancer and is related to immune infiltration.