高效液相色谱法对“黄金菊”茶中儿茶素和氨基酸组分含量的测定

HPLC Determination of Catechin and Amino Acid Components in Camellia sinensis‘Huangjinju’

为进一步研究江西特色茶树资源的生化成分,以“黄金菊”、安吉白茶、福鼎大白茶等茶树为试验材料,用高效液相色谱(high performance liquid chromatography,HPLC)法,对不同茶叶中儿茶素组分和氨基酸组分进行检测和对比分析。结果显示,“黄金菊”茶中儿茶素组分和氨基酸组分比较丰富,没食子酸酯儿茶素(gallate catechin,GC)、表没食子儿茶素(epigallocatechin,EGC)、儿茶素(catechin,C)、表儿茶素(epicatechin,EC)、表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)、没食子儿茶素没食子酸酯(gallocatechin gallate,GCG)、表儿茶素没食子酸酯(epicatechin gallate,ECG)、儿茶素没食子酸酯(catechin gallate,CG)含量分别为0.05%、1.21%、0.53%、0.99%、7.91%、0.23%、2.63%、0.01%;共检测出17种氨基酸组分,分别比安吉白茶、福鼎大白茶多检出1、3种氨基酸,检测出的成分含量与安吉白茶比较接近。试验结果说明,"黄金菊"茶树中儿茶素组分、氨基酸组分丰富,同时特征成分明显。

To study the biochemical characteristics of Camellia sinensis‘Huangjinju’in Jiangxi Province.The content of catechins and amiono acid were detected from Camellia sinensis‘Huangjinju’,Camellia sinensis‘Anjibaicha’,Camellia sinensis‘Fudingdabaicha’by high performance liquid chromatography(HPLC).The results show that the content of catechins and amino acids in Camellia sinensis‘Huangjinju’was rich.The contents of catechin gallate(GC),epigallocatechin(EGC),catechin(C),epicatechin(EC),epigallocatechin gallate(EGCG),gallocatechin gallate(GCG),epicatechin gallate(ECG)and catechin gallate(CG)in Camellia sinensis‘Huangjinju’were 0.05%,1.21%,0.53%,0.99%,7.91%,0.23%,2.63%and 0.01%respectively.17 kinds of amino acids were detected,one and three kinds of amino acids were more than Camellia sinensis‘Anjibaicha’and Camellia sinensis‘Fudingdabaicha’respectively,The components of Camellia sinensis‘Huangjinju’were close to Camellia sinensis‘Anjibaicha’.There are abundant catechins and amino acids in the“golden chrysanthemum”tea tree,and the characteristic components were obvious.