摘要
运用高效液相色谱四极杆飞行时间串联质谱(high-performance liquid chromatography coupled with quadrupole time-of-flight-mass spectrometry,HPLC-Q/TOF-MS)法对向日葵蜂花粉中的酚胺类化合物进行定性和定量分析。通过质谱数据解析和文献对照,向日葵蜂花粉中共鉴定出8种酚胺类化合物,其中4种为三-对-香豆酰亚精胺,4种为四-对-香豆酰精胺。定量分析显示,鉴定的酚胺类成分在限定质量浓度范围内线性关系良好。建立的HPLC-Q/TOFMS法能够对向日葵蜂花粉中的酚胺类成分进行准确、可靠分析。
The qualitative and quantitative analysis of phenolamides in sunflower bee pollen was carried out by high-performance liquid chromatography coupled with quadrupole time-of-flight-mass spectrometry(HPLC-Q/TOF-MS).By HPLC-Q/TOF-MS,a total of eight phenolamides including four kinds of tri-p-coumaroylspermidines and four kinds of tetra-p-coumaroylspermines were identified through mass spectrometric data analysis and comparison with literature.The regression equations showed a good linear relationship between peak areas and content of these compounds.The results showed that the HPLC-Q/TOF-MS method established could accurately and reliably analyse phenolamides in sunflower bee pollen.
作者
冯洲旭
张根生
孔令杰
乔江涛
肖兴英
张红城
FENG Zhou-xu;ZHANG Gen-sheng;KONG Ling-jie;QIAO Jiang-tao;XIAO Xing-ying;ZHANG Hong-cheng(Harbin University of Commerce,Harbin 150010,Heilongjiang,China;Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第5期177-183,共7页
Food Research and Development
关键词
高效液相色谱四极杆飞行时间串联质谱
向日葵蜂花粉
酚胺类化合物
定性分析
定量分析
high-performance liquid chromatography coupled with quadrupole time-of-flight-mass spectrometry(HPLC-Q/TOF-MS)
sunflower bee pollen
phenolamides
qualitative analysis
quantitative analysis
