紫花前胡素通过降低OPN表达抑制根尖周炎进程

Decursin Reduces Periapical Inflammation by Inhibiting OPN Expression

目的:探讨紫花前胡素对根尖周炎的影响及机制。方法:构建大鼠牙齿根尖周炎用药模型,分为正常对照组、模型观察组和加药处理组,用药处理的大鼠于第1周开始每天腹腔注射紫花前胡素溶液20 mg/kg或等量生理盐水,至第3周末处死。X线及HE染色观察病灶变化;免疫组化检测OPN表达情况。培养并用内毒素及紫花前胡素处理小鼠单核巨噬细胞RAW264.7,CCK-8法检测细胞增殖水平,ELISA法检测IL-6的表达,Western blot检测OPN的变化。结果:生理盐水处理组的大鼠根尖病灶面积明显大于紫花前胡素处理组,且紫花前胡素组大鼠组织中的OPN的表达降低。经内毒素处理的RAW264.7细胞增殖水平及IL-6和OPN的表达水平升高,而紫花前胡素抑制细胞增殖能力以及IL-6和OPN的表达。结论:紫花前胡素可以抑制OPN的表达,干预根尖周炎的炎症反应。

Objective:To study the effects and mechanism of decursin in periapical periodontitis.Methods:The rat periapical periodontitis model was established and divided into normal control group,model observation group,and apical periodontitis group.The rats in the apical periodontitis group were equally divided into the decursin group(given decursin,20mg/kg per day)and normal saline group(given the same dose of saline)in the first week,respectively.The rats were sacrificed in the third week.The area of the periapical lesions was analyzed by radiographs and HE staining.The expression of OPN in the alveolar bone was detected using immunohistochemical staining.Mouse mononuclear macrophages RAW264.7 cells were cultured and treated with LPS and decursin.The proliferation and the level of IL-6 were detected by CCK-8 assay and ELISA,respectively.The expression of OPN was examined by western blot.Results:The periapical lesion areas of the control group were remarkably higher than those of the decursin groups.Decursin decreased the protein levels of OPN.The expression of OPN,the proliferation ability,and the level of IL-6 of RAW264.7 cells were increased after treated with LPS.Decursin inhibited these effects.Conclusion:Decursin suppressed the expression of OPN and inhibited the progress of the periapical periodontitis.