优质食用紫薯南紫薯008脱毒繁育体系的构建

Construction of Virus-free Breeding System of High-quality Edible Purple Sweet Potato Nanzishu 008

甘薯病毒病是造成甘薯品种退化的主要原因,经种薯繁育代代累积,最终会导致绝收。以优质食用型紫薯品种南紫薯008为试验材料,进行了甘薯组培脱毒苗繁育体系的研究。结果表明:利用茎尖脱毒后的茎尖分生组织在含2mg/L 2,4-D的MS培养基中诱导胚性愈伤组织,建立胚性细胞悬浮培养系,经分化和再生,以提高扩繁率。将胚型细胞悬浮培养系建立的脱毒方式与水培快繁技术相结合,建立起一套完善的脱毒种薯种苗繁育体系,为四川丘陵地区脱毒种薯繁育提供了技术支持。

Sweet potato virus disease is the main reason for the degradation of sweet potato varieties,and it accumu⁃lates from generation to generation through seed potato breeding,which may eventually lead to no harvest.In this study,Nanzishu 008 which is a high-quality edible purple potato variety,was used as the material to study the sweet potato tissue culture virus-free seedling breeding system.The results showed that the stem apex meristem after the detoxification of the stem apex was used to induce embryogenic callus in MS medium containing 2mg/L 2,4-D,and the embryogenic cell suspension culture line was established,afterward differentiation and regeneration.It can great⁃ly increase the propagation rate.This combination of the detoxification method established by the embryonic cell sus⁃pension culture system and the hydroponic rapid propagation technology establishes a complete detoxification seed potato seedling breeding system.It provides a theoretical basis for formulating virus-free seed potato cultivation pro⁃cedures in Sichuan hilly areas.