基于蓝光光盘/光驱技术的生物分子嵌入式检测系统与仪器的研发

Research and Development of Biomolecular Embedded Detection System and Instrument Based on Blu-ray Disc/Drive Technology

针对蓝光技术的数字化分子检测方法存在的结果分析依赖于电脑、不适用于现场应用的问题,开展了采用嵌入式开发技术的定量检测系统及仪器的研发。通过嵌入式系统移植、光盘质量诊断软件移植和数据处理程序开发,实现了生物光盘的自动化数据分析和设备的小型化与集成化。通过在蓝光光盘表面设计墨点阵列实验,横向分辨率达到100μm,表明了该检测系统的可行性。通过制备生物素-链霉亲和素的生物光盘,利用本系统实现了链霉亲和素的自动化定量检测,进一步验证了本系统的可靠性和用于生物分子检测的可行性。

Although the current developed Blu-ray disc-based technology could realize digitized molecular detection,the result analysis of which depends on computer,not applicable for on-site applications.In order to overcome the above challenges,we herein report a quantitative detection system developed by the embedded technology.In particular,through the transplantation of embedded systems and disc quality diagnosis software,and the development of data processing programs,this system realizes the automatic analysis of bio-discs with this integrated device.It has been utilized for the detection of printed ink dots and the biotin-streptavidin binding reaction on BD surface,preving the reliability and feasibility for biomolecular detection.