健脾祛湿、凉血止血方对溃疡性结肠炎模型大鼠远期疗效的影响

Effect of Jianpi Qushi and Liangxue Zhixue recipe on the long-term efficacy of ulcerative colitis model rats

目的:观察健脾祛湿、凉血止血方对溃疡性结肠炎模型大鼠的远期治疗效果。方法:采用2,4,6-三硝基苯磺酸(2,4.6-TNBS)加免疫诱导法复制溃疡性结肠炎大鼠模型,52只大鼠随机分为中药高剂量组、中药低剂量组、柳氮磺吡啶(SASP)组、模型组。持续给药30 d后停药,停药30 d后观察大鼠结肠壁组织的病理学变化以及核因子κB(Nuclear Factor Kappa-B,NF-κB)p65、白细胞介素-10(Interleukin-10,IL-10)表达水平的变化。结果:中药高、低剂量组大鼠结肠黏膜损伤指数(Colon Macroscopic Damage Index,CMDI)评分均明显低于SASP组(P<0.05);中药高、低剂量组NF-κB p65表达均明显低于SASP组(P<0.05),中药高、低剂量组IL-10表达均明显高于SASP组(P<0.05)。结论:健脾祛湿、凉血止血方对实验性溃疡性结肠炎大鼠肠壁损伤有较好的远期治疗效果,其作用机制可能与降低NF-κB p65和提高IL-10表达水平有关。

Objective:To observe the effect of Jianpi Qushi and Liangxue Zhixue recipe(健脾祛湿、凉血止血方)on the long-term efficacy of ulcerative colitis model rats.Methods:The rat model of ulcerative colitis was reproduced by 2,4,6-nitrobenzenesulfonic acid(2,4,6-TNBS)and immune-induced method.52 rats were randomly divided into the high-dose Chinese medicine group,the low-dose Chinese medicine group,the salazosulfapyridine(SASP)group and the model group.After 30 days of continuous administration,the pathological changes of colon macoscopic damage index(CMDI)and NF-κB p65,IL-10 expression level were observed.Results:The CMDI of highdose and low-dose group was significantly lower than that of the SASP group(P<0.05).The expression of NF-κB p65 in high-dose and low-dose group was significantly lower than that of the SASP group(P<0.05).The expression of IL-I0 in high-dose and low-dose group was significantly higher than that in SASP group(P<0.05).Conclusion:Jianpi Qushi and Liangxue Zhixue recipe has better long-term efficacy on impaired colon of the experimental ulcerative colitis rats.Its mechanism may be related to reducing the expression of NF-κB p65 and boosting the level of IL-10.