知母-黄柏药对盐炙前后配伍对2型糖尿病大鼠糖脂代谢的影响

Effect of Compatibility of Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex Couplet Medicines on Glucolipid Metabolism in Type 2 Diabetic Rats Before and After Salt-processing

目的:考察知母-黄柏药对盐炙前后配伍对2型糖尿病(T2DM)大鼠糖脂代谢的影响。方法:采用高脂高糖饮食联合小剂量链脲佐菌素(STZ)制备T2DM大鼠模型,模型大鼠随机分为6组,分别为模型组,二甲双胍组(200 mg·kg^(-1)),生知母-生黄柏组,盐知母-盐黄柏组,生知母-盐黄柏组,盐知母-生黄柏组(6.48 g·kg^(-1));另设同周龄喂食普通饲料大鼠为空白组。各组大鼠按剂量连续灌胃给药30 d后,检测大鼠空腹血糖(FBG),空腹血清胰岛素(FINS),糖化血清蛋白(GSP),肝糖原含量,血脂[总胆固醇(TC),甘油三酯(TG),脂蛋白胆固醇(LDL-C/HDL-C)],游离脂肪酸(NEFA),脂肪细胞因子[瘦素、脂联素(ADP)],肾功能[血尿素氮(BUN)和肌酐(CRE)]等指标的变化,计算胰岛素敏感指数(ISI)与胰岛素抵抗指数(HOMA-IR);同时,采用苏木素-伊红(HE)染色观察大鼠胰腺、肝脏和肾脏组织形态学变化。结果:与模型组比较,知母-黄柏盐炙前后各配伍药对均能不同程度的降低T2DM大鼠FBG,GSP,TC,TG,LDL-C,NEFA,瘦素,BUN,CRE水平及HOMA-IR,升高FINS,HDL-C,ADP,肝糖原含量及ISI,且以盐知母-盐黄柏药对调节T2DM大鼠糖脂代谢的效果最为显著;各配伍药对均能一定程度的改善糖尿病大鼠胰腺、肝脏和肾脏组织病理学变化,其中盐知母-盐黄柏药对修复病理损伤的作用最佳。结论:知母-黄柏药对盐炙前后配伍对T2DM大鼠均有降糖调脂的作用,其中以盐知母-盐黄柏药对的降糖调脂综合药效最优。

Objective: To investigate the effect of compatibility of Anemarrhenae RhizomaPhellodendri Chinensis Cortex couplet medicines on glucolipid metabolism in type 2 diabetic rats before and after salt-processing. Method: The type 2 diabetic rat model was induced by high-fat and high-glucose diet combined with low dose streptozotocin(STZ),the model rats were randomly divided into six groups,including the model group, metformin group(200 mg·kg^(-1)),and different compatibility groups of raw and salt-processed of Anemarrhenae Rhizoma and Phellodendri Chinensis Cortex(6.48 g·kg^(-1)). In addition,The same week old rats fed with normal diet were set as the blank group. After 30 d of continuous intragastric administration,changes of fasting blood glucose(FBG),fasting serum insulin(FINS),glycosylated serum protein(GSP),hepatic glycogen,blood lipid[total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDLC), high density lipoprotein cholesterol(HDL-C)], nonesterified fatty acid(NEFA), adipocytokines[adiponectin(ADP)and leptin)],kidney function[blood urea nitrogen(BUN)and creatinine(CRE)]and other indicators of rats from different groups were detected,and the insulin sensitivity index(ISI)and insulin resistance index(HOMA-IR) were calculated, hematoxylin-eosin(HE) staining was used to observe the morphological changes of pancreas,liver and kidney of rats from different groups. Result: Compared with the model group,compatibility of Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex couplet medicines before and after salt-processing all could decrease the levels of FBG,GSP,TC,TG,LDL-C,NEFA,leptin,BUN,CRE and HOMA-IR,and increase the contents of FINS,HDL-C,ADP,hepatic glycogen and ISI,among which the compatibility of salt-processed Anemarrhenae Rhizoma and salt-processed Phellodendri Chinensis Cortex had the most significant effect on regulating glucolipid metabolism in type 2 diabetic rats. The compatibility of all couplet medicines could improve the histopathological changes of pancreas,liver and kidney in type 2 diabetic rats,among which the compatibility of salt-processed Anemarrhenae Rhizoma and saltprocessed Phellodendri Chinensis Cortex had the most prominent effect on repairing pathological damage.Conclusion: The compatibility of Anemarrhenae Rhizoma and Phellodendri Chinensis Cortex before and after salt-processing can improve glucolipid metabolism in type 2 diabetic rats,while the comprehensive effect of saltprocessed Anemarrhenae Rhizoma and salt-processed Phellodendri Chinensis Cortex on lowering glucose and regulating lipid is the best.