蛋白质翻译后修饰的质谱鉴定方法和在精准医学研究中的应用

Identification of protein post-translational modificationsand application in precision medical research by mass spectrometry

近年来,随着科学技术的发展,质谱法(MS)作为一种创新型分析技术应运而生。MS适用于大量样品的分析,其凭借高效、灵敏、准确、稳定以及便捷等特性,成为蛋白质组学应用的首要选择,在蛋白质分析领域中得到了广泛应用。蛋白质组学对于疾病的早期诊断、预后和监测疾病的发展至关重要,其中,蛋白质翻译后修饰(PTM)是蛋白质功能调控的重要方式,因此,关于PTM的检测在实验过程中非常重要。本文综述了泛素化、类泛素化、乙酰化、糖基化等PTM质谱检测以及本实验室建立的新型类泛素化质谱检测方法,并探讨了PTM质谱鉴定在精准医学研究领域的应用情况。

In recent years,with the development of science and technology,mass spectrometry(MS)has emerged as an innovative analytical technique.MS is suitable for a large number of analyses and is widely used in the field of protein analysis.It has become the main choice for proteomics applications with its efficiency,sensitivity,accuracy,stability and convenience.Proteomics is essential for early stage diagnosis,prognosis and monitoring of disease development.Among them,the protein post-translational modification(PTM)is an important way of regulating protein functionand.The testing of PTM is very important in the experimental process.This article reviews mass spectrometry analysis of PTM(ubiquitination,sumoylation,acetylation,glycosylation)andthe new sumoylation mass spectrometry detection method defined in our laboratory.This article also discusses the application of mass spectrometry in precision medicine.