玉米赤霉烯酮时间分辨荧光试纸条的制备及特性研究

Preparation and Properties of Time-Resolved Fluorescent Strip for Zearalenone Detection

研究制备一种基于时间分辨荧光免疫层析法的玉米赤霉烯酮(ZEN)快速定量检测试纸条。采用铕纳米微球作为荧光探针,标记抗ZEN的单克隆抗体,应用竞争抑制原理建立免疫层析定量方法并对其进行方法学考核。结果表明:ZEN荧光试纸条的灵敏度为0.1 ng/mL,线性范围为0.1~5.0 ng/mL,玉米、小麦阴性样品的加标回收率在93.45%~112.20%之间,对于同一个样品5次重复测定的变异系数小于15%。ZEN快速定量检测试纸条具有灵敏度高、反应时间短、准确定量、稳定性好等技术特点,适用于谷物及制品中玉米赤霉烯酮的快速定量检测。

The purpose of this study was to prepare a kind of Zearalenone fast quantitative test strip based on time-resolved fluorescence immunochromatographic assay.Europium nanoparticles were used as fluorescent probes to label anti-zearalenone monoclonal antibodies,and a quantitative immunochromatography method was established based on competitive inhibition principle.The results showed that the sensitivity of Zearalenone fluorescent detection strip was 0.1 ng/mL,the linear range was 0.1~5.0 ng/mL,the standard recovery rate of negative samples of corn and wheat was between 93.45%and 112.2%,and the coefficient of variation of 5 repeats for the same sample was less than 15%.The results show that the Zearalenone fluorescent test strip has the technical characteristics of high sensitivity,short reaction time and accurate quantification,which is suitable for the rapid quantitative detection of Zearalenone in grain and its products.