优质小米三级中红外光谱研究

Research on Quality Millet by the Three-step MIR Spectroscopy

采用中红外(MIR)光谱分别开展山东、山西和河北等地五种小米的光谱特征研究。结果表明:小米的红外吸收模式主要包括OH伸缩振动模式(νOH)、CH3不对称伸缩振动模式(νasCH3)、CH2不对称伸缩振动模式(νasCH2)、CH2对称伸缩振动模式(νsCH2)、CH伸缩振动模式(νCH)、C==O伸缩振动模式(νC==O)、酰胺Ⅰ带吸收模式(ν^(amide-Ⅰ))、酰胺Ⅱ带吸收模式(ν^(amide-Ⅱ))、酰胺Ⅲ带吸收模式(ν^(amide-Ⅲ))及C—O伸缩振动模式(νC—O);进一步以小米νasCH3-二维、νasCH2-二维、νsCH2-二维和νCH-二维为研究对象,小米相应的同步2D-MIR光谱存在着一定的差异性,可以快速准确地鉴别上述五种小米。研究拓展了三级MIR光谱技术在优质小米结构及快速鉴别的研究范围。

The structures of five kinds of millet from Shandong,Shanxi,Hebei and other provinces were studied by mid infrared(abbreviation:MIR)spectroscopy.The results showed that the infrared absorption modes of millet mainly include OH stretching vibration mode(νOH),CH3 asymmetrical stretching vibration mode(νasCH3),CH2 asymmetrical stretching vibration mode(νasCH2),CH2 symmetrical stretching vibration mode(νsCH2),CH stretching vibration mode(νCH),C==O stretching vibration mode(νC==O),protein amideⅠband mode(ν_(amide-Ⅰ)),protein amideⅡband mode(ν_(amide-Ⅱ)),protein amideⅢband mode(ν_(amide-Ⅲ))and C—O stretching vibration mode(νC—O).Furthermore,the corresponding synchronous 2D-MIR spectroscopy of millet,includingνasCH3-2d,νasCH2-2d,νsCH2-2d andνCH-2d,have some differences,which can quickly and accurately identify the five kinds of millets.This paper expanded the research scope of three-step MIR spectroscopy in structure and rapid identification of high-quality millet.