广泛性焦虑对心肌梗死大鼠心房及外周血心房利钠肽水平的影响

Effects of Generalized Anxiety on ANP Concentration in Atrium and Peripheral Blood of Rats with Myocardial Infarction

目的探讨焦虑对心肌梗死后心房及外周血心房利钠肽(ANP)水平的影响。方法选取SPF级6周龄健康雄性SD大鼠22只,采用不确定空瓶刺激建立焦虑模型,与冠状动脉左前降支结扎建立心肌梗死模型相结合建立心肌梗死合并焦虑大鼠模型,根据随机数字法将SD大鼠分为假手术组、心肌梗死组及心肌梗死合并焦虑组。超声心动图评价大鼠心功能,行为学试验评价大鼠焦虑状态,酶联免疫吸附试验(ELISA)检测外周血ANP含量,免疫印迹法(Western Blotting)检测大鼠心房ANP表达。结果心肌梗死组、心肌梗死合并焦虑组左心室射血分数及左室短轴缩短率低于假手术组,左室收缩末期内径、左室舒张末期内径大于假手术组,差异均有统计学意义(P<0.01);与假手术组相比,心肌梗死合并焦虑组水平运动格子数及垂直运动次数明显减少(P<0.05);高架十字迷宫试验显示;与假手术组相比,心肌梗死组也更倾向于待在闭臂里,开臂次数(OE)/[OE+闭臂次数(CE)]与开臂停留时间(OT)/[OT+闭臂停留时间(CT)]比值均明显下降,差异均有统计学意义(P<0.05);ELISA结果显示,与假手术组相比,心肌梗死组及心肌梗死合并焦虑组大鼠外周血ANP浓度均明显升高,差异均有统计学意义(P<0.01)。Western Blotting结果显示,与假手术组相比,心肌梗死组及心肌梗死合并焦虑组ANP及其受体表达含量均明显下降,差异均有统计学意义(P<0.05);与心肌梗死组相比,心肌梗死合并焦虑组ANP及其受体表达含量均明显上升,差异均有统计学意义(P<0.05)。结论焦虑刺激可使心肌梗死大鼠外周血及心房ANP表达增加。

Objective To investigate the effect of anxiety on the concentration of atrial natriuretic peptide(ANP)in atrium and peripheral blood after myocardial infarction.Methods SD rats were divided into sham operation group,myocardial infarction group,and myocardial infarction combined with anxiety group according to random number method.An anxiety model was established using uncertain empty bottle stimulation,and rat model of myocardial infarction combined with anxiety was established in combination with a model of myocardial infarction by ligation of the left anterior descending coronary artery.Echocardiography was used to evaluate the heart function of rats.Behavioral tests were used to evaluate the anxiety of rats.Enzyme-linked immunosorbent assay(ELISA)was used to detect the content of ANP in peripheral blood.Western Blotting was used to detect the expression of ANP in rat atria.Results The left ventricular ejection fraction of myocardial infarction group and myocardial infarction combined with anxiety group were lower than that of sham operation group,the difference was statistically significant(P<0.01);The horizontal exercise score and vertical movement of the myocardial infarction combined with anxiety group significantly reduced than those in sham operation group.The elevated cross-maze test was more likely to stay in the closed arm(both P<0.05).Compared with sham operation group,the rats in myocardial infarction group also more inclined to stay in the closed arm,and the ratios of open arm entry(OE)/[OE+closed arm entry(CE)]and open arm entry time(OT)/[OT+closed arm entry time(CT)]significantly decreased(P<0.05).ELISA results showed that compared with sham operation group,the peripheral blood ANP concentration in myocardial infarction group and myocardial infarction combined with anxiety group significantly increased,the difference was statistically significant(P<0.01).Western Blotting results showed that compared with sham operation group,the expression levels of ANP and its receptors in myocardial infarction group and myocardial infarction combined with anxiety group significantly reduced,and the difference was statistically significant(P<0.05).Compared with myocardial infarction group,the expression levels of ANP and its receptors in myocardial infarction combined with anxiety group significantly increased,and the difference was statistically significant(P<0.05).Conclusion Anxiety stimulation can increase the expression of ANP in peripheral and atrium of myocardial infarction rats.