白芨种子萌发及无菌苗移栽成活影响因素研究

Study on Influencing Factors of Seed Germination and Aseptic Seedling Transplanting Survival of Bletilla striata

探讨了不同培养基和培养方式对白芨种子无菌萌发的影响、不同尺寸育苗袋对白芨无菌苗移栽成活的影响以及遮荫强度对白芨容器苗生长状况的影响。结果表明:不同培养基及培养方式处理的白芨种子萌发率差异不显著,但固体BJ4培养基中的球茎大于其他处理。育苗袋以较大规格(16 cm×20 cm)处理的无菌苗平均成活率及生长量较好。就遮荫强度而言,四针的遮阳网更利于白芨容器苗的成活和生长,移栽两个月后成活率为60%,平均株高为5.65 cm,而无遮荫状态不适合白芨容器苗的生长。

The effects of different media and culture methods on aseptic germination of Bletilla striata seeds,the effects of different size seedling bags on the survival of Bletilla striata aseptic seedlings,and the influence of shade intensity on the growth of Bletilla striata container seedlings were studied.The results showed that there was no significant difference in germination rate of Bletilla striata seeds in different media and culture methods,but the corm in solid BJ4 medium was larger than that other treatments;the average survival rate and growth of the aseptic seedlings treated with larger size(16 cm×20 cm)seedling bags were better.In terms of shading strength,the four-needle shading net was more conducive to the survival and growth of Bletilla striata.The survival rate of Bletilla striata was 60%and the average seedling height was 5.65 cm after transplanting for 2 months.However,the unshaded state was not suitable for the growth of Bletilla striata container seedlings.