以线粒体为靶点筛选中药抗肝毒性的活性成分研究思路与方法

Research ideas and method on screening active components of traditional Chinese medicine against hepatotoxicity with mitochondria as target

肝脏是药物代谢的主要场所,肝细胞线粒体是药物肝损伤的重要靶标,线粒体自噬可以维持细胞内线粒体的健康运转以及细胞的稳定增殖,因此利用线粒体自噬来清除受损线粒体是抗药物性肝损伤的重要策略。多种中药中含有可以增强线粒体自噬的活性成分,可以调节线粒体自噬从而缓解相关疾病。但如何准确高效地从中药中发现并评价此类对线粒体有靶向活性成分的研究方法鲜有报道。而采用液质联用技术,以体内过程结合血清药理学的方法能准确、高效地锁定作用于线粒体靶点的中药活性成分。该文综述了增加线粒体自噬解药物肝毒性的中药成分研究思路与方法,为以线粒体为靶点的中药活性成分研究提供新思路与方法。

Liver is the main place of drug metabolism.Mitochondria of hepatocytes are important targets of drug-induced liver injury.Mitochondrial autophagy could maintain the healthy operation of mitochondria in cells and the stable proliferation of cells.Therefore,the use of mitochondrial autophagy to remove damaged mitochondria is an important strategy of anti-drug-induced liver injury.Active ingredients that could enhance mitochondrial autophagy are contained in many traditional Chinese medicines,which could regulate the mitochondrial autophagy to alleviate relevant diseases.However,there are only a few reports on how to accurately and efficiently identify and evaluate such components targeting mitochondria from traditional Chinese medicine.Liquid chromatography-mass spectro-metry(LC-MS)combined with serum pharmacology in vivo can be used to accurately and efficiently find active ingredients of traditional Chinese medicine acting on mitochondrial targets.This paper reviewed the research ideas and methods of traditional Chinese medicine ingredients for increasing the hepatotoxicity of mitochondrial autophagy,in order to provide new ideas and methods for the study of active ingredients of traditional Chinese medicine targeting mitochondria.