象皮生肌膏促糖尿病大鼠皮肤溃疡愈合机制研究

Study on the Mechanism of Xiangpi Shengji Ointment(象皮生肌膏)in Promoting Skin Ulcer Healing in Diabetic Rats

目的:探讨象皮生肌膏促糖尿病大鼠皮肤溃疡愈合的作用机制。方法:SPF级雄性SD大鼠36只,随机选取24只大鼠建立糖尿病大鼠模型,造模成功后随机分为模型组和治疗组各12只,另取12只大鼠作为正常组。所有大鼠均建立缺损性皮肤溃疡大鼠模型,治疗组用象皮生肌膏外敷溃疡创面,对照组和模型组仅用生理盐水清洗创面。药物干预第14天时分别取3组创面组织,通过HE染色观察组织大体结构,免疫组化染色观察创面血管化程度,酶联免疫反应技术测定组织中血管内皮生长因子(VEGF)含量。比较3组于干预后3、7、14、21 d创面愈合率,评价创面愈合情况。结果:HE染色显示治疗组大鼠较模型组细胞活力增强,皮肤各层结构层次清晰。免疫组化染色显示治疗组大鼠较模型组有更多的血管生成,但略少于正常组。与模型组比较,治疗组大鼠干预后14、21 d时组织VEGF平均表达量明显升高(P<0.05)。治疗组大鼠于干预后第3天创面闭合率与模型组比较无明显差异(P>0.05),但治疗组大鼠于干预后7、14、21 d创面闭合率明显高于模型组(P<0.05)。结论:象皮生肌膏外敷可恢复高糖受损细胞活力,促使细胞增殖,促进溃疡组织VEGF表达,加速血管形成,改善局部血运,促进创面上皮化,从而促进糖尿病皮肤溃疡创面愈合。

Objective:To explore the mechanism of Xiangpi Shengji Ointment in promoting skin ulcer healing in diabetic rats.Methods:A total of 36 SPF male SD rats,24 rats were randomly selected to establish a diabetic rat model.After successful modeling,they were randomly divided into model group and treatment group,with 12 rats in each group,and another 12 rats were taken as the normal group.All rats established rat models of defective skin ulcers.The treatment group was externally applied with Xiangpi Shengji Ointment to the wounds of the ulcers,while the control group and model groups were only used to clean the wounds with saline.On the 14 thday of drug intervention,the wound tissues of the three groups were taken respectively.The general structure of the tissue was observed by HE staining,the degree of vascularization of the wound was observed by immunohistochemical staining,and the content of vascular endothelial growth factor(VEGF)in the tissue was determined by enzyme-linked immunoreaction technique.The wound healing rates of the three groups at 3,7,14 and 21 days after intervention were compared to evaluate the wound healing.Results:HE staining showed that the rats in the treatment group had stronger cell viability than the model group,and the skin structure was clear.Immunohistochemical staining showed that rats in the treatment group had more angiogenesis than the model group,but slightly less than the normal group.Compared with the model group,the average expression of VEGF in the tissues of rats in the treatment group increased significantly at 14 and 21 days after intervention(P<0.05).The wound closure rate of rats in the treatment group was not significantly different from that of the model group on the third day after intervention(P>0.05),but the wound closure rate of rats in the treatment group was significantly higher than that of the model group at 7,14,and 21 days after intervention(P<0.05).Conclusion:External application of Xiangpi Shengji Ointment can restore the viability of high-glucosedamaged cells,promote cell proliferation,promote VEGF expression in ulcer tissue,accelerate blood vessel formation,improve local blood supply,promote wound epithelization,and promote healing of diabetic skin ulcers.