期刊文献+

铵离子对多杀菌素发酵生产的影响 被引量:1

Effect of ammonium ion on the production of spinosad fermentation
下载PDF
导出
摘要 通过研究铵离子对多杀菌素生产的影响,优化发酵条件,提高多杀菌素产量。结果表明,当培养基中添加0.75 g·L^(-1)的硫酸铵时,多杀菌素产量提高了9.2%。在此基础上,通过Plackett-Burman设计确定了葡萄糖、棉籽蛋白和硫酸铵是对多杀菌素产量影响最大的3个因素。经响应面优化后,多杀菌素产量为80.7 mg·L^(-1),较初始产量提高了13.7%。随后,使用优化培养基进行5 L罐分批和补料分批发酵,多杀菌素产量为90.5 mg·L^(-1)和158.3 mg·L^(-1),较摇瓶发酵分别提升了12.1%和96.2%。 To increase the yield of spinosad,we studied the effect of ammonium ion on the production of spinosad,and optimized the fermentation conditions.The results show that when 0.75 g·L^(-1) ammonium sulfate was added to the medium,the yield of spinosad was increased by 9.2%.Based on this,glucose,cottonseed protein and ammonium sulfate were identified by Plackett-Burman design as factors that had the greatest influence on the yield of spinosad.Furthermore,after the application of response surface methodology optimization,the yield of spinosad was 80.7 mg·L^(-1),which was 13.7%higher than the initial yield.Finally,using the optimized medium for batch and fed-batch fermentation in a 5 L fermenter,the yields of spinosad were 90.5 mg·L^(-1) and 158.3 mg·L^(-1),which were increased by 12.1%and 96.2%,respectively,compared with that of shake flask fermentation.This study shows that the addition of low concentration of ammonium sulfate can effectively promote the production of spinosad.
作者 张松 扶教龙 徐敏强 汪佳琪 张瀚 胡翠英 白净 ZHANG Song;FU Jiaolong;XU Minqiang;WANG Jiaqi;ZHANG Han;HU Cuiying;BAI Jing(School of Chemistry and Life Sciences,SUST,Suzhou 215009,China)
出处 《苏州科技大学学报(自然科学版)》 CAS 2021年第1期64-70,84,共8页 Journal of Suzhou University of Science and Technology(Natural Science Edition)
基金 苏州市科技计划项目(SNG2017053) 苏州科技学院科研基金资助项目(XKZ201411)。
关键词 铵离子 多杀菌素 PLACKETT-BURMAN设计 响应面优化法 刺糖多孢菌 ammonium ion spinosad Plackett-Burman design response surface methodology Saccharopolyspora spinosa
  • 相关文献

参考文献16

二级参考文献164

共引文献71

同被引文献12

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部