铵离子对多杀菌素发酵生产的影响

Effect of ammonium ion on the production of spinosad fermentation

通过研究铵离子对多杀菌素生产的影响,优化发酵条件,提高多杀菌素产量。结果表明,当培养基中添加0.75 g·L^(-1)的硫酸铵时,多杀菌素产量提高了9.2%。在此基础上,通过Plackett-Burman设计确定了葡萄糖、棉籽蛋白和硫酸铵是对多杀菌素产量影响最大的3个因素。经响应面优化后,多杀菌素产量为80.7 mg·L^(-1),较初始产量提高了13.7%。随后,使用优化培养基进行5 L罐分批和补料分批发酵,多杀菌素产量为90.5 mg·L^(-1)和158.3 mg·L^(-1),较摇瓶发酵分别提升了12.1%和96.2%。

To increase the yield of spinosad,we studied the effect of ammonium ion on the production of spinosad,and optimized the fermentation conditions.The results show that when 0.75 g·L^(-1) ammonium sulfate was added to the medium,the yield of spinosad was increased by 9.2%.Based on this,glucose,cottonseed protein and ammonium sulfate were identified by Plackett-Burman design as factors that had the greatest influence on the yield of spinosad.Furthermore,after the application of response surface methodology optimization,the yield of spinosad was 80.7 mg·L^(-1),which was 13.7%higher than the initial yield.Finally,using the optimized medium for batch and fed-batch fermentation in a 5 L fermenter,the yields of spinosad were 90.5 mg·L^(-1) and 158.3 mg·L^(-1),which were increased by 12.1%and 96.2%,respectively,compared with that of shake flask fermentation.This study shows that the addition of low concentration of ammonium sulfate can effectively promote the production of spinosad.