不同激素处理对复色紫薇扦插生根效果的影响

Effects of different hormone treatments on rooting of polychromatic crape myrtle cuttings

【目的】筛选复色紫薇扦插的最优激素处理方法,旨在促进复色紫薇生产效率的提高。【方法】以复色紫薇嫩枝作为插穗,分析不同激素种类、激素浓度、处理时间、处理部位下复色紫薇生根率和根系效果指数,并检测最优处理和清水浸泡的插穗生根过程中内源激素和营养物质变化。【结果】激素处理能够明显改善复色紫薇生根情况,激素种类和激素浓度对生根率和根系效果指数均有显著影响(P<0.05),处理时间仅对生根率影响显著(P<0.05),处理部位影响均不显著(P>0.05)。四因素的综合影响为激素种类>激素浓度>处理时间>处理部位。最优处理为400 mg/L的IBA处理枝条下1/3部分5 min。对最优处理和清水浸泡的插穗生根过程中内源激素和营养物质进行检测发现,激素处理能够增加生根关键期的IAA含量、可溶性糖含量和可溶性蛋白含量,降低ABA含量和ZR含量,促进根原基的分化和不定根的伸长。【结论】复色紫薇扦插在400 mg/L的IBA处理枝条下1/3部分5 min效果较优,能够获得较高的生根率和根系效果指数。

【Objective】To screen the optimal hormone treatment method for polychromatic crape myrtle cuttings for improving the production efficiency of polychromatic crape myrtle.【Methods】The young branches of polychromatic crape myrtle were used as cuttings to analyze rooting rate and root effect index under different hormone types,hormone concentrations,treatment time,treatment part,and to detect its endogenous hormones and nutrients changes during the rooting process of cuttings under the optimal treatment and water soaking.【Results】The hormone treatment could improve polychromatic crape myrtle rooting significantly.Hormone types and hormone concentration both had a highly significant effect on the rooting rate and root effect index(P<0.05),and treatment time could affect significantly the rooting rate only(P<0.05),but reatment part had no significant difference(P>0.05).The comprehensive influence of four factors was hormone types>hormone concentrations>treatment time>treatment part.The optimal treatment was 400 mg/L IBA to treat the lower 1/3 part of the branches for 5 min.The detection of endogenous hormones and nutrients during the rooting process of cuttings that were optimally treated and soaked in water showed that hormone could increase the content of IAA,soluble sugar content and soluble protein content in the critical period of rooting,meanwhile reduce the ABA and ZR content,and promote differentiation of root primordia and elongation of adventitious roots.【Conclusion】The cutting effect of 400 mg/L IBA on the lower 1/3 part of branches for 5 min was better,and the higher rooting rate and root effect index of polychromatic crape myrtle could be obtained.