软骨组织工程中种子细胞选择的研究热点

Research hotspot of seed cell selection in cartilage tissue engineering

背景:耳、鼻、喉、气管与关节等软骨缺损修复始终是临床治疗所面临的难点之一,软骨组织工程的出现,成为了软骨修复的热门发展方向,其中种子细胞是构建组织工程软骨的关键。目的:文章拟对不同类型的种子细胞特点及其在软骨组织工程中的应用、存在的问题等研究进展做一综述,为软骨组织工程从实验向临床进行转化带来新的思路。方法:第一作者通过检索中国知网、万方、PubMed、Scopus和Web of Science数据库的相关文献,以“种子细胞,组织工程,软骨细胞,干细胞,共培养”为中文检索词,以“seed cells,tissue engineering,chondrocytes,stem cells,co-culture”为英文检索词进行检索。最终选取符合标准的70篇文献进行综述。结果与结论:①目前软骨组织工程的实验研究取得了大量的成果,但关于种子细胞的选择问题仍是一大难题,软骨细胞、各种类型的间充质干细胞、胚胎干细胞、诱导多能干细胞等,均具有不同程度的优势,同时也面临着不同的挑战;②各种种子细胞的发现、发展与应用均推动了软骨组织工程的进步,其中软骨细胞与干细胞共培养是目前比较热门且具有研究前景的种子细胞选择。

BACKGROUND:The repair of cartilage defects such as ears,noses,throats,trachea and joints has always been one of the difficulties faced by clinical treatment.The emergence of cartilage tissue engineering has become a popular development direction for cartilage repair,in which seed cells are used to construct tissue engineered cartilage key.OBJECTIVE:This article intends to review the characteristics of various types of seed cells,their application in cartilage tissue engineering,and existing problems,and to bring new ideas to clinical transformation.METHODS:The first author searched the relevant articles of CNKI,Wanfang,PubMed,Scopus and Web of Science databases,using“seed cells;tissue engineering;chondrocytes;stem cells;co-cultivation”as the Chinese and English search terms.Finally,70 articles that met the criteria were selected for review.RESULTS AND CONCLUSION:(1)Current experimental research on cartilage tissue engineering has achieved a lot of results,but the problem of selecting seed cells is still a big problem.Chondrocytes,various types of mesenchymal stem cells,embryonic stem cells,and induced pluripotent stem cells have different degrees of advantages,but also face different challenges.(2)The discovery,development and application of various seed cells have promoted the progress of cartilage tissue engineering.Among them,the method of co-culture of chondrocytes and stem cells is currently a popular and promising seed choice for research.