基于微量元素分析技术的中药板蓝根和大青叶营养转运模式研究

Studies on Nutrient Transport Model of Isatidis Radix and Isatidis Folium Based on Trace Elements Analysis Technology

采用电感耦合等离子体质谱(ICP-MS)和电感耦合等离子体发射光谱(ICP-OES)技术,测定不同生长时间的板蓝根和大青叶中的微量元素含量。其中,采用ICP-MS法测量Cr、Co、La、Ce、Pr、Nd、Sm、Eu、Gd、Dy、Li、Be、V、Ga、U、Nb、Zr、Bi、Th、Er、Yb、Y、Sc元素,采用ICP-OES法测量Fe、Al元素。结果表明,不同生长时间的板蓝根和大青叶中,24种微量元素含量的变化趋势存在一定的相似性。板蓝根中,24种微量元素的含量在7~9月达到最低值,之后逐渐增加,11月达到最高值;大青叶中,24种微量元素的含量均在8月和11月达到最高值。根据微量元素含量的变化结果,初步推断大青叶的最佳采收时期为8月和11月,板蓝根为11月,可与大青叶同时采摘。本方法简单、快速、精密度和准确性好,为板蓝根和大青叶营养转运模式的研究奠定基础。

Inductively coupled plasma mass spectrometry(ICP-MS)and inductively coupled plasma optical emission spectrometry(ICP-OES)technologies were used to determine the contents of trace elements in Isatidis Radix and Isatidis Folium at different growth times.Among them,the contents of trace elements including Cr,Co,La,Ce,Pr,Nd,Sm,Eu,Gd,Dy,Li,Be,V,Ga,U,Nb,Zr,Bi,Th,Er,Yb,Y,and Sc were measured by ICP-MS,while the elements Fe and Al were measured by ICP-OES.According to the test results,the trend of the contents of 24 trace elements in Isatidis Radix and Isatidis Folium at different growth times indicated certain similarity.The contents of 24 trace elements in Isatidis Radix reached the lowest value between July and September,then gradually increased and reached the highest value in November.The contents of those elements in Isatidis Folium all reached the highest point in August and November.Based on the results of trace element content changes,the preliminary inference was made that the optimal harvest time for Isatidis Folium is August and November,and the optimal harvest time for Isatidis Radix is November,which means that Isatidis Folium and Isatidis Radix can be plucked at the same time.The method is easy and fast,has good precision and accuracy which to some extent lays a foundation for the study of nutrient transport patterns of Isatidis Radix and Isatidis Folium.