金堂黑山羊不同年龄、性别及不同温湿度指数下的血液生化指标测定

Determination of blood biochemical indexes of Jintang black goats at different ages,genders and temperature humidity index

为了测定金堂黑山羊不同年龄、性别及在较高和较低温湿度指数(THI)下的血液生化指标,为环境调控及饲养管理提供依据,试验定时定点测定山羊舍内干湿球温度,计算THI。随机选取金堂黑山羊48只,其中1,2,3岁3个年龄段各16只,公母各半(母羊空怀),分别在THI为85.96,83.44,69.04条件下,颈静脉采血,测定血液肾上腺素(EPI)、生长激素(GH)、甲状腺素(T4)、丙氨酸氨基转移酶(ALT)、γ-谷氨酰转移酶(γ-GT)、碱性磷酸酶(ALP)、天门冬氨酸氨基转移酶(AST)、胆碱酯酶(CHE)、总胆红素(T-Bil)、直接胆红素(D-Bil)、总蛋白(TP)、白蛋白(ALB)、总胆汁酸(TBA)和葡萄糖(Glu)等14项指标,并按照年龄、性别及不同THI整理血液生化指标并进行比较分析。结果表明:随着年龄增大,ALT活性及TP、ALB、TBA浓度显著或极显著升高(P<0.05或P<0.01),ALP活性和Glu浓度极显著降低(P<0.01)。母山羊GH浓度显著高于公山羊(P<0.05),T4浓度和ALP活性极显著高于公山羊(P<0.01),γ-GT活性极显著低于公山羊(P<0.01)。在较高THI条件下,EPI和T4及GH浓度较高,其中THI为83.44和85.96时三者的浓度均极显著高于THI为69.04时的浓度(P<0.01)。THI为69.04与85.96时,相应ALB浓度、γ-GT活性前者高于后者,而T-Bil、D-Bil及TBA浓度则是后者高于前者。说明ALT和ALP活性及TP、ALB、TBA和Glu浓度与年龄有关;ALP和γ-GT活性及GH和T4浓度与性别有关;在较高THI条件下,EPI和T4浓度揭示金堂黑山羊处于热应激状态,同时GH浓度较高,ALT和AST等主要肝功能指标活性下降。

In order to determine the blood biochemical indexes of Jintang black goats at different ages, genders and at higher and lower temperature humidity index(THI), and to provide basis for environmental regulation and feeding management, The dry and wet bulb temperatures in the goat house were measured at fixed points and the THI was calculated in the experiment. 48 Jintang black goats were randomly selected for the experiment, and there were 16 goats in each age groups of 1, 2 and 3 years old, with half male and half female(the female goats were nonpregnant). Under the conditions of THI of 85.96, 83.44 and 69.04, blood was collected from the jugular vein, and 14 indexes such as serum adrenaline(EPI), growth hormone(GH), thyroxine(T4), alanine aminotransferase(ALT), γ-glutamyl transferase(γ-GT), alkaline phosphatase(ALP), aspartate aminotransferase(AST), cholinesterase(CHE), total bilirubin(T-Bil), direct bilirubin(D-Bil), total protein(TP), albumin(ALB), total bile acid(TBA) and glucose(Glu) were measured according to the age, sex and different THI and serum biochemical indexes were compared and analyzed. The results showed that with the increase of age, the ALT activigy and the concentration of TP,ALB and TBA increased(P<0.05 or P<0.01), while the ALP activity and the concentration of Glu decreased(P<0.01). The GH concentration of female goats was significantly higher than that of male goats(P<0.05), the concentrations of T4 and ALP activity were significantly higher than that of male goats(P<0.01), and the γ-GT activity was significantly lower than that of male goats(P<0.01). Under higher THI condition, the concentrations of EPI, T4 and GH were higher, and the concentrations at THI 83.44 and 85.96 were significantly higher than those at THI 69.04(P<0.01). When THI was 69.04 and 85.96, the concentrations of ALB and γ-GT activity were higher in the former than in the latter, while the concentrations of T-BIL, D-BIL and TBA were higher in the latter. This showed that ALT, ALP activity and TP, ALB, TBA, and Glu concentrations were related to age. The ALP, γ-GT activity and GH, T4 concentration were related to gender. Under higher THI conditions, EPI and T4 levels revealed that Jintang black goats were under heat stress, while GH levels were high, and major liver function indexes such as ALT and AST were decreased.