长白猪体内牛磺酸合成关键酶及其转运体的表达

Expression of key enzymes of taurine biosynthesis and their transporter in tissues of Landrace

为了研究牛磺酸合成关键酶及其转运体(taurine transporter,TAUT)在长白猪肝脏及内分泌腺中的表达情况,试验随机选取120日龄长白猪,公母各半,取肝脏、下丘脑、垂体、卵巢、睾丸、甲状腺及肾上腺,采用RT-PCR方法、实时荧光定量RT-PCR方法及Western-blot技术对这些组织/器官中牛磺酸合成关键酶——半胱亚磺酸脱羧酶(cysteine sulfinate decarboxylase,CSD)、半胱氨酸双加氧酶(cysteine dioxygenase,CDO)及牛磺酸转运体(taurine transporter,TAUT)基因mRNA及蛋白表达情况进行了测定。结果表明:长白猪肝脏和内分泌腺中均存在CSD、CDO及TAUT基因mRNA表达,其中CSD基因mRNA表达水平由高到低的顺序为甲状腺、垂体、肝脏、卵巢、下丘脑、肾上腺、睾丸,CDO基因mRNA表达水平由高到低的顺序为睾丸、卵巢、甲状腺、肝脏、垂体、下丘脑、肾上腺,TAUT基因mRNA表达水平由高到低的顺序为垂体、肝脏、卵巢、下丘脑、甲状腺、肾上腺、睾丸;长白猪肝脏和内分泌腺均可表达CSD、CDO及TAUT蛋白。说明长白猪肝脏、下丘脑、垂体、卵巢、睾丸、甲状腺及肾上腺均可通过CDO-CSD途径生物合成牛磺酸,牛磺酸可能与这些组织/器官的功能发挥密切相关。

To explore the expression of key enzymes of taurine biosynthesis and their transporter in liver and endocrine glands of Landrace,120-day-old Landrace were randomly selected for the experiment, males and females were equal numbers. Their liver, hypothalamus, pituitary, ovary, testis, thyroid gland and adrenal gland were collected. The mRNA and protein expression of cysteine sulfinate decarboxylase(CSD), cysteine dioxygenase(CDO) and tavrine transporter(TAUT) genes were detected by RT-PCR, qRT-PCR and Western-blot. The results showed that CSD, CDO and TAUT mRNA in liver and endocrine gland and adrenal gland of Landrace were detected. The expression levels of CSD mRNA in the tissues from high to low were thyroid gland, pituitary, liver, ovary, hypothalamus, adrenal gland and testis. The CDO mRNA expression levels in the tissues were testis>ovary>thyroid gland>liver>pituitary>hypothalamus>adrenal gland. The TAUT mRNA expression levels in the tissues were pituitary>liver>ovary>hypothalamus>thyroid gland>adrenal gland > testis. The CSD, CDO and TAUT proteins were expressed in liver and endocrine glands of Landrace. The results indicated that taurine could be synthesized in liver, hypothalamus, pituitary, ovary, testis, thyroid and adrenal gland of Landrace, and taurine might play an important role in these tissues.