口服热灭活草分枝杆菌对鸡新城疫和禽流感H9亚型疫苗效果影响的研究

Study on the effect of oral heat-killed Mycobacterium phlei on Newcastle disease and Avian influenza H9 subtype vaccine

为了研究热灭活草分枝杆菌作为口服非特异性免疫调节剂对鸡新城疫(ND)和禽流感H9亚型(AI-H9)疫苗效果的影响,试验采用完全随机法将150只1日龄雏鸡分成5组,分别为空白对照组、试验组(分为草分枝杆菌高、中、低剂量组)和疫苗对照组,试验组分别在3日龄时人工灌服热灭活草分枝杆菌,连续7 d,除空白对照组外,其余各组在7日龄时皮下注射鸡新城疫、禽流感(H9亚型)二联灭活疫苗,在免疫后7,14,21,28,35天分别测定鸡只平均体重、血清和小肠黏液IgA含量、外周淋巴细胞增殖情况和HI抗体水平。结果表明:在免疫后14,21,28,35天,小肠黏液IgA、外周淋巴细胞OD570值显著高于空白对照组和疫苗对照组(P<0.05);在免疫后21,28,35天,鸡只体重、血清IgA显著高于空白对照组和疫苗对照组(P<0.05);免疫后试验组ND、AI-H9抗体水平与空白对照组和疫苗对照组相比均无显著差异(P>0.05)。说明口服热灭活草分枝杆菌能够显著提升细胞免疫应答,显著提高血清和小肠黏液IgA分泌量,促进外周淋巴细胞增殖,提高鸡只平均体重。

In order to study the effect of heat-killed Mycobacterium phlei as an oral non-specific immunomodulator on Newcastle disease(ND) and Avian influenza H9 subtype(AI-H9) vaccine, A total of 150 one-day-old chicks were divided into 5 groups using the completely randomized method in this experiment,including blank control group,test groups(with high,medium and low doses of Mycobacterium phlei),and vaccine control group. The test groups were perfused artificially with heat-killed Mycobacterium phlei on 3 days of age for 7 consecutive days. Except for the blank control group,the other groups were injected subcutaneously with the combined inactivated vaccine of Newcastle disease and Avian influenza(H9 subtype) on 7 days old. On 7,14,21,28,35 days post immunization,the average body weights and IgA contents in sera and small intestinal mucus,peripheral lymphocyte proliferation,HI antibody levels in chickens were determined respectively. The results showed that on 14,21,28,35 days post immunization,IgA contents in small intestinal mucus,and OD570 values of peripheral lymphocytes were significantly higher than those in blank control group and vaccine control group(P<0.05). On 21,28,35 days post immunization,the body weights and serum IgA contents were significantly higher than those in blank control group and vaccine control group(P<0.05). The ND and AI-H9 antibody levels in the test group were not significantly different from those in blank control group and vaccine control group(P>0.05). The results suggested that oral heat-killed Mycobacterium phlei could significantly enhance the cellular immune response,significantly increase serum and small intestinal mucus IgA secretion,promote the proliferation of peripheral lymphocytes,and increase the average weights of chickens.