决明子蒽醌苷对非酒精性脂肪肝病大鼠脂酸β氧化相关酶蛋白表达的影响

Effects of Anthraquinone Glycosides from Juemingzi on the Expression of Fatty AcidβOxidation Related Enzyme Protein in Rats with Nonalcoholic Fatty Liver Disease

目的:探讨决明子蒽醌苷对非酒精性脂肪肝病(nonalcoholic fatty liver disease,NAFLD)大鼠脂酸β氧化途径关键酶过氧化物酶酰基辅酶A氧化酶1(peroxidase acyl coenzyme A oxidase 1,ACOX1)和肉毒碱棕榈酰转移酶I(carnitine palmitoyl transferase 1A,CPT1A)表达的影响。方法:将60只SD大鼠适应性喂养10天后,随机分为正常组、模型组、阳性药物(多烯磷脂酰胆碱)组及决明子蒽醌苷低、中、高剂量组,每组10只,高脂高糖饮食8周成功构建NAFLD模型,给予相应治疗8周后处死大鼠,收集大鼠血清和肝脏,检测大鼠血脂、肝功能、肝脏病理学以及肝脏组织中ACOX1和CPT1A的mRNA和蛋白表达水平。结果:与正常组相比,模型组大鼠肝功能指标谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)、血脂指标甘油三酯(triglyceride,TG)和胆固醇(total cholesterol,TC)均升高(P<0.05);与模型组比较,决明子蒽醌苷3个剂量组和阳性药物组肝功能及血脂指标均降低(P<0.05),高剂量组低于阳性药物组(P<0.05)。HE染色正常组大鼠肝脏细胞大小均一,分布均匀,模型组出现明显的脂肪空泡和炎症反应,决明子蒽醌苷3个剂量组和阳性药物组均可以减少肝脏脂肪浸润。与正常组比较,ACOX1和CPT1A、模型组及决明子蒽醌苷3个剂量组和阳性药物组均降低(P<0.05);与模型组比较,决明子蒽醌苷3个剂量组和阳性药物组ACOX1和CPT1A均升高(P<0.05),高剂量组高于阳性药物组(P<0.05)。结论:决明子蒽醌苷可以通过增加脂酸β氧化途径关键酶ACOX1和CPT1A mRNA以及蛋白表达水平,促进肝脏脂肪颗粒排出,最终改善肝功能。

Objective:To explore the effects of anthraquinone glycoside from Juemingzi(semen cassiae)on the expressions of CPT1A and ACOX1,key enzymes of fatty acid beta oxidation pathway in rats with NAFLD.Methods:After adaptive feeding for ten days,sixty SD rats were randomized into the normal group,the model group,positive medicine group(polyene phosphatidylcholine),low,moderate and high dosages groups of anthraquinone glycoside from Juemingzi,ten rats each group,NAFLD models were successfully established after giving high fat and sugar diet for eight weeks,the rats were sacrificed after eight weeks,to collect the serum and liver of the rats,to detect the expressions of ACOX1 and CPT1A mRNA and protein in liver tissue,liver pathology,liver function and blood lipid.Results:Compared with the normal group,the levels of ALT,AST,TG and TC of the rats in the model group rose notably(P<0.05);compared with the model group,the indexes of liver function and blood lipid in high,moderate and low dose groups of anthraquinone glycoside and positive medicine group lowered notably(P<0.05),high dose group was lower than positive medicine group notably(P<0.05),liver cells were uniform in size and distribution in HE staining normal group,fat vacuoles and inflammatory reaction were observed in the model group,liver fatty infiltration relieved in three doses groups and positive medicine group.Compared with the normal group,ACOX1 and CPT1A lowered notably in the model group,three dose groups of anthraquinone glycoside,positive medicine group(P<0.05),compared with the model group,ACOX1 and CPT1A rose in three dose groups and positive medicine group notably(P<0.05),high dose group was higher than positive medicine group(P<0.05).Conclusion:Anthraquinone glycoside from Juemingzi could promote the discharge of liver fatty granules,eventually improve liver function through increasing the expressions of ACOX1 and CPT1A mRNA and protein.