微酸性电解水实验室微生物杀灭效果研究

Microbial killing efficacy of slightly acidic electrolyzed water in laboratory

目的研究某微酸性电解水实验室微生物杀灭效果。方法选取某次氯酸水发生器现制微酸性电解水,测定不同有效氯含量、不同作用时间其对细菌繁殖体、芽孢以及真菌的杀灭效果。结果该微酸性电解水有效氯含量为34.3~118 mg/L,有机干扰物牛血清白蛋白浓度为3.0%和0.3%时,分别作用1.0、3.0、5.0、10.0 min,对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌的平均杀灭对数值均>5.00,对白假丝酵母菌的平均杀灭对数值均>4.00;有效氯含量为101~118 mg/L,牛血清白蛋白浓度为0.3%时,分别作用5.0、10.0、30.0 min,对枯草杆菌黑色变种芽孢的平均杀灭对数值均>5.00。结论在特定实验条件下,该微酸性电解水对细菌的繁殖体、芽孢以及真菌均达到消毒剂消毒合格标准。

Objective To study microbial killing efficacy of slightly acidic electrolyzed water(SAEW)in laboratory.Methods SAEW produced by hypochlorous acid water generator was selected to determine the microbial killing efficacy of different available chlorine concentration and different action time on bacterial propagules,spores and fungi.Results When the available chlorine concentration in SAEW was 34.3-118 mg/L,concentration of organic interfering substance bovine serum albumin(BSA)were 3.0%and 0.3%,the average killing logarithm values for Staphylococcus aureus,Escherichia coli,and Pseudomonas aeruginosa were all>5.00 after 1.0,3.0,5.0,10.0 minute contact time respectively,the average killing logarithm values for Candida albicans were all>4.00;when available chlorine concentration was 101-118 mg/L and concentration of BSA was 0.3%,the average killing loga-rithm values for spores of Bacillus subtilis var.niger were all>5.00 after 5.0,10.0 and 30.0 minute contact time respectively.Conclusion Under specific experimental conditions,SAEW can meet the disinfection qualified stan-dard of disinfectant for bacterial propagules,spores and fungi.