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微小RNA-615-3p下调色素框同源蛋白6对肺癌吉西他滨化疗抵抗细胞株的影响及其机制 被引量:2

Effect of microRNA-615-3p on gemcitabine resistant cell lines of lung cancer by regulating chromobox 6 and its mechanism
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摘要 目的研究微小RNA-615-3p(miR-615-3p)通过调控色素框同源蛋白6(CBX6)对肺癌细胞吉西他滨敏感性的影响及其机制。方法将肺癌吉西他滨化疗抵抗细胞株A549/GEM分为4组:空白组、吉西他滨(GEM)组、GEM转染-1组和GEM转染-2组。分别在A549/GEM中转染anti-miR-615-3p和Si-CBX6后,用GEM处理48 h。用噻唑蓝检测A549/GEM细胞增殖活力(OD值);流式细胞术检测A549/GEM细胞凋亡率;蛋白质印迹法检测CBX6蛋白表达水平。结果空白组、GEM组、GEM转染-1组在2 d时的增殖活力分别为1.43±0.10,1.02±0.09和0.63±0.06,GEM组与空白组比较,或GEM转染-1组与GEM组比较,差异均有统计学意义(P<0.05,P<0.01)。GEM组、GEM转染-1组和GEM转染-2组的CBX6蛋白相对表达分别为1.02±0.09,1.66±0.09和1.21±0.11;这3组的胞凋亡率分别为(12.18±1.08)%,(19.28±1.65)%和(13.81±1.21)%;这3组的在2 d时的增殖活力分别为0.91±0.07,0.64±0.05和0.88±0.11。GEM转染-1组与GEM组比较,或GEM转染-2与GEM转染-1组比较,差异均有统计学意义(P<0.05,P<0.01)。结论敲降miR-615-3p通过上调CBX6表达,促进肺癌细胞对GEM的敏感性。 Objective To investigate the effect of microRNA-615-3 p(miR-615-3 p) on gemcitabine sensitivity of lung cancer cells by regulating Chromobox 6(CBX6) and its mechanism.Methods Lung cancer gemetabine chemotherapeutic cell line A549/GEM was divided into 4 groups:blank group, gemetabine(GEM) group, GEM+ transfection-1 group, GEM+ transfection-2 group.After transfection of anti-miR-615-3 p or Si-CBX6 in A549/GEM respectively,the cells were treated with GEM for 48 h.The proliferation activity(ODvalue)of A549/GEM cells was detected by Thiazolam.The apoptosis rate of A549/GEM cellsdetected by flow cytometry.Western blotting was used to detect expression level of CBX6 protein.Results The proliferation activity of the blank group, GEM group and GEM+ transfection-1 group on Day 2 were 1.43±0.10, 1.02±0.09 and 0.63±0.06, respectively.Comparison between GEM group and blank group, or between GEM+ transfection-1 group and GEM group,the difference of the factors were significant(P < 0.05,P < 0.01).The relative expression of CBX6 protein in GEM group,GEM + transfection-1 group and GEM + transfection-2 group were 1.02 ± 0.09,1.66 ± 0.09 and 1.21 ± 0.11,respectively;the apoptosis rates in the three groups were(12.18 ± 1.08) %,(19.28 ± 1.65) % and(13.81 ± 1.21) %,respectively;the proliferation activity in the three groups were 0.91 ± 0.07,0.64 ± 0.05 and0.88 ± 0.11 respectively.Comparison between GEM + transfection-1 group and GEM group,or between GEM +transfection-2 group and GEM + transfection-1 group,the difference of the factors were statistically significant(P < 0.05,P < 0.01).Conclusion Knockdown of miR-615-3 p increased the sensitivity of lung cancer cells to gemcitabine by up-regulating the expression of CBX6.
作者 段彤 李劲松 DUAN Tong;LI Jin-song(Department of Emergency,First Hospital of Changsha,Changsha 410005,Hunan Prorince,China;Department of Orthopedics,The Third Xiangya Hospital,Central South University,Changsha 410000,Hunan Province,China)
出处 《中国临床药理学杂志》 CAS CSCD 北大核心 2021年第4期416-420,共5页 The Chinese Journal of Clinical Pharmacology
基金 国家自然科学基金青年科学基金资助项目(81502331)。
关键词 微小RNA-615-3p 色素框同源蛋白6 肺癌 吉西他滨 敏感性 microRNA-615-3p chromobox 6 lung cancer gemcitabine sensitivity
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