大米ACE抑制肽制备工艺优化和生物活性研究

Optimization of Preparation and Bioactivity of Rice ACE Inhibitory Peptides

采用胰蛋白酶从大米蛋白中酶解制备血管紧张素转化酶(ACE)抑制肽,在单因素实验的基础上,采用响应面法(RSM)对工艺条件进行了优化。在最佳酶解条件下,大米蛋白水解液ACE抑制率可达75.17%。酶解产物经大孔树脂脱盐,97.99%的大米ACE抑制肽的分子质量主要分布在1000 U以下,大米ACE抑制肽的IC_(50)值为1.5710 mg/mL。经MALDI-TOF/TOF-MS分析进一步鉴定了目标肽的结构,活性肽的氨基酸组成确定为Val-Pro-Phe-Arg-Pro(VPFRP)。采用大米ACE抑制肽进行细胞实验,观察大米ACE抑制肽对人脐静脉内皮细胞(HUVECs)培养及内皮素-1(ET-1)释放的影响,结果表明,大米ACE抑制肽可通过抑制内皮细胞产生ET-1而改善高血压。

To optimize the preparation of angiotensin converting enzyme(ACE)inhibitory peptide from rice protein,the technical conditions were optimized by response surface methodology(RSM)on the basis of single factor experiments.The ACE inhibition rate of rice protein hydrolysate could reach 75.17%with the optimum conditions of enzymatic hydrolysis.The enzymatic hydrolysate was desalted through the macroporous resin,while 97.99%of the molecular weight of rice ACE inhibitory peptides were less than 1000 U,and the IC_(50)value of rice ACE inhibitory peptide was 1.5710 mg/mL.The structure of the target peptide was further identified by MALDI-TOF/TOF-MS analysis,and the amino acid composition of the active peptide was determined as Val-Pro-Phe-Arg-Pro(VPFRP).The rice ACE inhibitory peptide was used for cell experiments to observe its effects on the culture of human umbilical vein endothelial cells(HUVECs)and endothelin-1(ET-1)release.The results showed that the rice ACE peptides could improve hypertension by its ability to inhibit ET-1 production from endothelial cells.