疏风解毒胶囊对急性咽炎大鼠干预作用及机制探讨

Intervention effect and mechanism of Shufeng Jiedu capsule on acute pharyngitis rats

目的探讨疏风解毒胶囊对急性咽炎大鼠的干预作用及可能作用机制,为其临床应用提供理论依据。方法将70只健康SD大鼠随机分为7组,除正常组外,其余组大鼠均采用咽部喷雾氨水法制备急性咽炎模型,连续3 d。从第4天开始,克林霉素组给予克拉霉素缓释片20 mg/(kg·d)灌胃,疏风解毒胶囊高、中、低剂量组分别给予疏风解毒胶囊2.2 g/(kg·d)、1.1 g/(kg·d)、0.55 g/(kg·d)灌胃,正常组及模型组给予等量的蒸馏水灌胃,均2次/d,连续5 d。观察并记录各组大鼠的一般情况及症状表现;实验结束后取腹主动脉血,采用ELISA法检测各组大鼠血清前列腺素E_(2)(PGE_(2))、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平。结果造模3 d后,模型组大鼠咽部黏膜红肿充血明显,状态萎靡,血清中PGE2、IL-1β、TNF-α水平明显高于正常组(P均<0.05)。灌胃2 d后,克拉霉素组、疏风解毒胶囊各组大鼠咽部黏膜红肿充血均有不同程度减轻,疏风解毒胶囊高剂量组和克拉霉素组灌胃5 d后基本痊愈。灌胃结束后,克拉霉素组、疏风解毒胶囊各组大鼠血清PGE2、IL-1β、TNF-α水平均明显低于模型组(P均<0.05),疏风解毒胶囊高剂量组和克拉霉素组均明显低于疏风解毒胶囊中、低剂量组(P均<0.05),但疏风解毒胶囊高剂量组明显高于克拉霉素组(P均<0.05)。结论疏风解毒胶囊可以有效减轻急性咽炎症状体征,作用机制可能与下调血清PGE2、IL-1β、TNF-α水平有关。

Objective It is to explore the interventional effect of Shufeng Jiedu Capsule on rats with acute pharyngitis and its possible mechanism,so as to provide a theoretical basis for its clinical application.Methods Seventy healthy SD rats were randomly divided into 7 groups.Except for the normal group,the rats in the other groups were all used to prepare acute pharyngitis models by spraying ammonia from the pharynx for 3 consecutive days.From the 4th day,the clindamycin group was given clarithromycin sustained-release tablets 20 mg/(kg·d)by gavage,and the Shufeng Jiedu capsule high,medium and low dose groups were respectively given Shufeng Jiedu capsule 2.2 g/(kg·d),1.1 g/(kg·d),0.55 g/(kg·d)by gavage,the normal group and model group were given the same amount of distilled water by gavage,all 2 times/d,for 5 consecutive days.The general conditions and symptoms of rats in each group were observed and recorded;at the end of the experiment,the blood from the abdominal aorta of rats in each group was taken,and the serum levels of prostaglandin E_(2)(PGE_(2)),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)were detected by ELISA method.Results After 3 days of modeling,the pharyngeal mucosa of the rats in the model group was obviously red and swollen and congested,and they were listless,the serum levels of PGE2,IL-1β,and TNF-αwere significantly higher than those of the normal group(all P<0.05).After 2 days of intragastric administration,the swelling and congestion of the pharyngeal mucosa of the rats in the clarithromycin group and Shufeng Jiedu capsule groups were relieved more or less.The rats in the high-dose Shufeng Jiedu capsule group and the clarithromycin group were basically cured after 5 days of intragastric administration.After gavage,the levels of serum PGE2,IL-1β,and TNF-αof rats in the clarithromycin group and Shufeng Jiedu capsule groups were significantly lower than those in the model group(all P<0.05),and the Shufeng Jiedu capsule high-dose group and the clarithromycin group was significantly lower than the Shufeng Jiedu capsule middle and low dose groups(P<0.05),but the Shufeng Jiedu capsule high dose group was significantly higher than the clarithromycin group(P<0.05).Conclusion Shufeng Jiedu capsules can effectively alleviate the symptoms and signs of acute pharyngitis.The mechanism of action may be related to the down-regulation of serum levels of PGE2,IL-1β,and TNF-α.