泪液特异性IgA检测诊断单纯疱疹病毒性角膜炎的初步研究

Determination of herpes simplex virus specific IgA in tears for diagnosis of herpes simplex keratitis:a preliminary study

目的评估泪液单纯疱疹病毒(HSV)特异性IgA(HSV-IgA)在单纯疱疹病毒性角膜炎(HSK)的临床应用价值。方法采用毛细管收集105例门诊确诊的HSK患者(77例上皮染色组及28例上皮完好组)和73例对照组患者的泪液,采用酶联免疫吸附试验(ELISA)法检测泪液中HSV-IgA抗体水平,并进行受试者工作特征曲线(ROC曲线)分析。结果泪液HSV-IgA的敏感度为72.38%,特异度为84.93%;患病眼的HSVIgA水平要显著高于未患病及对照组(P<0.0001);上皮染色组HSV-IgA水平要显著高于上皮完好组(P=0.0027)。结论泪液HSV-IgA检测可以作为HSK诊断的一个有效参考指标。

Objective To evaluate the clinical value of tear herpes simplex virus(HSV) special IgA in HSK. Methods HSV special IgA in tears was detected in 105 HSK patients consist of 77 fluorescence staining positive and 28 negative, and 73 controls by enzyme linked immunosorbent assay, then the results were analyzed by receiver operator characteristic curve. Results The sensitivity and specificity were 72.38% and 84.93% respectively. HSV-IgA level in sick eyes of HSK patients was significantly higher than in healthy eyes and controls(P<0.0001). Fluorescence staining positive HSK patients had a higher tear HSV-IgA level(P=0.0027). Conclusions Determinating HSV-IgA in tears had referential value in diagnosing HSK.