miR-145通过调控MMP-2、MMP-9的表达对卵巢癌细胞调亡、增殖、迁移的影响

Effect of miR-145 on apoptosis,proliferation and migration of ovarian cancer cells by regulating the expression of MMP-2 and MMP-9

目的研究微小RNA-145(miR-145)通过调控基质金属蛋白酶(MMP)-2、MMP-9的表达对卵巢癌细胞调亡、增殖、迁移的影响。方法将卵巢癌细胞株随机分为3组,其中对照组为单纯卵巢癌细胞株,不做其他处理,进行正常培养;miR-145组为含miR-145质粒的慢病毒转染的卵巢癌细胞株;NC组为含NC质粒的慢病毒转染的卵巢癌细胞株。检测3组卵巢癌细胞的凋亡、增殖、迁移情况。检测3组MMP-2、MMP-9蛋白和mRNA表达水平。结果与对照组、NC组比较,miR-145组卵巢癌细胞凋亡率明显升高,卵巢癌细胞增殖数、迁移数明显下降,差异均有统计学意义(P<0.05);对照组与NC组卵巢癌细胞凋亡率、增殖数、迁移数差异无统计学意义(P>0.05)。miR-145组MMP-2、MMP-9蛋白和mRNA表达水平均低于NC组、对照组,差异均有统计学意义(P<0.05)。对照组与NC组MMP-2、MMP-9蛋白和mRNA表达水平差异无统计学意义(P>0.05)。结论miR-145可抑制卵巢癌细胞的增殖、迁移,促进调亡,其作用机制可能与miR-145能降低MMP-2、MMP-9的表达有关。

Objective To study the effects of microRNA-145(miR-145)on the apoptosis,proliferation and migration of ovarian cancer cells by regulating the expression of matrix metalloproteinase(MMP)-2 and MMP-9.Methods The ovarian cancer cell lines were randomly divided into 3 groups,among which the control group was a simple ovarian cancer cell line,without other treatments,and was cultured normally.MiR-145 group was ovarian cancer cell line transfected by lentivirus containing miR-145 plasmid.NC group was ovarian cancer cell line transfected by lentivirus containing NC plasmid.The apoptosis,proliferation and migration of ovarian cancer cells in 3 groups were detected.The protein and mRNA expression levels of MMP-2,MMP-9 in 3 groups were detected.Results Compared with control group and NC group,the apoptosis rate of ovarian cancer cells significantly increased,and the number of proliferation and migration of ovarian cancer cells significantly decreased in miR-145 group,the differences were statistically significant(P<0.05).There was no significant difference on the apoptosis rate,proliferation number and migration number of ovarian cancer cells between control group and NC group(P>0.05).The expression levels of MMP-2,MMP-9 protein and mRNA in miR-145 group were lower than those in NC group and control group,the differences were statistically significant(P<0.05).There was no significant difference on the expression levels of MMP-2 and MMP-9 protein and mRNA between control group and NC group(P>0.05).Conclusion MiR-145 can inhibit the proliferation and migration of ovarian cancer cells,promote apoptosis,and its mechanism may be related to reducing the expression levels of MMP-2 and MMP-9.