云南7个地区女性HPV亚型感染情况调查

Research of HPV subtype infection in 7 areas of Yunnan

目的分析云南7个地区女性人乳头瘤病毒(HPV)亚型感染情况,为宫颈癌防治提供参考依据。方法收集2018年1月至2019年12月于昆明医科大学第一附属医院以及云南其他6个地区共7624例进行HPV基因分型检测的女性为研究对象。用PCR-反向点杂交法进行HPV基因分型检测,分析云南7个地区女性HPV亚型感染情况。结果7624例受检者中,检出HPV阳性1225例,阳性率为16.07%,其中高危型HPV阳性967例,所占比例最高,为78.94%;单一亚型阳性970例,所占比例最高,为79.18%。HPV阳性率最高的3种HPV亚型依次为HPV52(17.90%)、HPV16(13.48%)、HPV58(11.59%)。<20岁女性的HPV阳性率最高,为36.62%(26/71),不同年龄段女性HPV阳性率比较,差异有统计学意义(χ^(2)=36.557,P<0.05)。7个地区HPV阳性率比较,差异有统计学意义(χ^(2)=27.959,P<0.05)。各月份HPV阳性率比较,差异无统计学意义(χ^(2)=15.319,P>0.05)。春季HPV阳性率最高,为17.90%;其次是冬季,为16.15%;秋季最低,为14.98%,各季节HPV阳性率比较,差异无统计学意义(χ^(2)=6.757,P>0.05)。结论云南7个地区女性有较高的H PV阳性率,HPV52、HPV16、HPV58型是主要的感染亚型,HPV感染以高危型、单一亚型为主。

Objective To analyze the infection of human papillomavirus(HPV)subtype in women in 7 areas of Yunnan,and to provide reference for the prevention and treatment of cervical cancer.Methods From January 2018 to December 2019,a total of 7624 women who underwent HPV genotyping in the First Affiliated Hospital of Kunming Medical University and other 6 areas of Yunnan were selected as the research objects.HPV genotypes were detected by PCR-reverse dothybridization,and analyzed the infection of HPV subtypes in 7 areas of Yunnan.Results Among 7624 subjects,1225 cases were HPV positive,with a positive rate of 16.07%.Among them,967 cases of high-risk HPV positive,accounting for the highest proportion,was 78.94%,970 cases of single subtype positive,accounting for the highest proportion,was 79.18%.The 3 HPV subtypes with the highest HPV positive rate were HPV52(17.90%),HPV16(13.48%)and HPV58(11.59%).The highest positive rate of HPV was 36.62%(26/71)in women under 20 years old.There was significant difference in the positive rate of HPV among different age groups(χ^(2)=36.557,P<0.05).The difference of HPV positive rate in 7 areas was statistically significant(χ^(2)=27.959,P<0.05).There was no significant difference in HPV positive rate among different months(χ^(2)=15.319,P>0.05).The positive rate of HPV was the highest in spring(17.90%),followed by winter(16.15%),and the lowest in autumn(14.98%).There was no significant difference in the positive rate of HPV among different seasons(χ^(2)=6.757,P>0.05).Conclusion Females in 7 areas of Yunnan have a higher HPV positive rate.HPV52,HPV16 and HPV58 are the main infection subtypes,and HPV infection is mainly high-risk and single subtype.