大孔树脂分离红花籽粕酶解产物制备抗氧化肽的工艺研究

Preparation of Antioxidant Peptides from Enzymatic Hydrolysis of Safflower Seed Meal by Macroporous Adsorption Resin

为制备红花籽粕抗氧化活性肽,比较不同酶解工艺下产物的抗氧化性,研究AB-8大孔树脂分离工艺,对比分离前后多肽抗氧化性,结果表明:碱性蛋白酶Alcalase酶解产物抗氧化性最佳,测得还原力为1.755,DPPH自由基清除率39.84%,羟自由基清除率26.76%、超氧阴离子自由基清除率25.90%,多肽含量达到10.71 mg/mL;选择AB-8树脂分离,采用上样流速3 BV/h、上样量24 mL、80%乙醇洗脱、洗脱流速1.00 mL/min工艺分离,且AB-8分离后样品的DPPH自由基清除率、羟自由基清除率、超氧阴离子自由基清除率均有增强。

For safflower seed meal antioxidant active peptide,compared different enzyme hydrolysis product oxidation resistance and research AB-8 macroporous resin separation process,the contrast peptides oxidation resistance,the results showed that the enzymatic hydrolysis product of Alcalase had the best antioxidant property,its reducing ability was 1.755,DPPH free radical clearance rate 39.84%,hydroxyl free radical clearance rate 26.76%,ultra oxygen anion clearance rate 25.90%,peptide content was 10.71 mg/mL;AB-8 resin was selected for separation,and the peak effect of enzymatic hydrolysate was best when the sample flow rate was 3 BV/h,the sample volume was 24 mL,the eluent concentration was 80%ethanol,and the elution flow rate was 1.00 mL/min.After AB-8 separation,DPPH radical clearance,hydroxyl radical clearance and superoxide anion clearance were enhanced.