模拟退火算法色差校正的傅里叶叠层显微彩色成像技术

Fourier Ptychographic Microscopy Color Imaging with Simulated-annealing Chromatic Aberration Correction

提出一种基于模拟退火算法的自动聚焦方法,可以分别重建三种照明色光的高分辨率图像,从而实现高分辨率彩色图像的构建。模拟仿真和分辨率靶及生物切片样品实验结果均表明,即使色差较严重的显微系统,利用该方法也能准确地获得高分辨率彩色图像,极大降低了对显微镜的性能要求,扩展了傅里叶叠层彩色成像的适用范围。

An auto focusing method based on simulated annealing algorithm is presented to recover the respective high resolution images for three different illumination colors,thus to recover the high resolution color image.Simulation modeling as well as the experiments on resolution target and biological slices all demonstrate that the high resolution color image can be recovered even for the microscopic system with serious chromatic aberration,which significantly relaxes the request for high quality microscope,thus expanding the application scope of the Fourier ptychographic microscopy.