新型肿瘤免疫抑制靶点唾液酸结合免疫球蛋白样凝集素-15的生物信息学预测

Bioinformatics prediction of sialic acid binding Ig like lectin-15 as a novel tumor immunosuppressive target

目的预测唾液酸结合免疫球蛋白样凝集素-15(sialic acid binding Ig like lectin-15,SIGLEC-15)的生物学信息。方法应用多种生物信息学工具对SIGLEC-15的理化性质、组织表达特异性、亚细胞定位、空间结构、翻译后修饰位点、蛋白相互作用等进行预测,同时建立SIGLEC-15的系统进化树,并进行分析。结果 SGLEC-15是一种碱性不稳定蛋白,具有亲水性,含有信号肽结构。SGLEC-15具有1个跨膜结构域,主要定位于细胞外(34.80%)、细胞膜(30.40%)、内质网(21.70%)和高尔基体(13.00%),在正常细胞及癌细胞中均有表达。SGLEC-15的高级结构弹性较大,拥有2个N-糖基化位点,1个O-糖基化位点及8个可能的蛋白磷酸化位点。SGLEC-15与TYROBP等4个蛋白具有高置信度的相互作用关系。人类SIGLEC-15与猩猩、白狨猴等灵长类动物SIGLEC-15的同源性最高,在脊椎动物进化过程中相对保守。结论 SIGLEC-15可作为肿瘤免疫抑制的靶点,为进一步肿瘤免疫疗法的研究奠定了基础。

Objective To predict the bioinformatics of sialic acid binding Ig like lectin-15(SIGLEC-15). Methods The physicochemical properties,tissue expression specificity,subcellular localization,spatial structure,post-translational modification site and protein interaction were predicted by a variety of bioinformatics tools,while a phylogenetic tree of SIGLEC-15 was plotted and analyzed. Results SGLEC-15 was an unstable basic protein with hydrophilicity and with signal peptide structure,which had a transmembrane domain mainly distributed in extracellular region(34. 80%),cell membrane(30. 40%),endoplasmic reticulum(21. 70%)and Golgi body(13. 00%),and was expressed in both normal and cancer cells. SGLEC-15,with high structural flexibility,had two N-glycosylation sites,one O-glycosylation site and eight possible protein phosphorylation sites,as well as a high confidence interaction with four proteins such as TYROBP.The SGLEC-15 from human origin showed high homology to those from primates such as chimpanzee and white marmoset,which was relatively conserved during evolution of vertebrates. Conclusion SIGLEC-15 may be used as a tumor immunosuppressive target,which lays a foundation of further study on immunotherapy of tumors.