CYP19A1、STS基因在黔北麻羊不同组织中的表达

Expression of CYP19A1,STS Genes in Different Tissues of Qianbei Ma Goat

为探究CYP19A1、STS基因在黔北麻羊下丘脑、垂体、子宫、输卵管、卵巢5个性腺组织中的表达水平。本试验以黔北麻羊为研究对象,提取下丘脑、垂体、子宫、输卵管、卵巢5个性腺组织的RNA并逆转录合成cDNA第一链,采用实时荧光定量PCR(q-PCR)技术检测CYP19A1、STS基因的m RNA在黔北麻羊5个性腺组织中的表达水平。结果表明CYP19A1、STS基因的mRNA在黔北麻羊5个性腺组织中均有不同程度的表达,且2个基因在卵巢中的表达量相对最高,在下丘脑中的表达量相对最低,两者之间差异极显著(p<0.01)。CYP19A1基因的mRNA在黔北麻羊5个性腺组织中的表达量由高到低依次为:卵巢>子宫>输卵管>垂体>下丘脑;STS基因的m RNA在黔北麻羊5个性腺组织中的表达量由高到低依次为:卵巢>垂体>子宫>输卵管>下丘脑。本研究结果为今后进一步探讨CYP19A1、STS基因对山羊的遗传机理提供了研究依据。

In order to explore the expression levels of CYP19 A1 and STS genes in the five gonad tissues of the hypothalamus,pituitary,uterus,oviduct and ovaries in the Qianbei Ma goats.In this study,Qianbei Ma goats was taken as the research object.The RNA was extracted from five gonad tissues comprising the hypothalamus,pituitary,uterus,oviduct and ovary,and reversed transcribed to the first strand of cDNA.The mRNA of CYP19 A1 and STS genes was detected by real-time quantitative PCR(q-PCR)expression levels in five individual gonad tissues.The results reveal that the mRNAs of CYP19 A1 and STS genes were expressed in different degrees in the five gonad tissue of Qianbei Ma goats.The expression of the two genes in the ovary was relatively highest and the expression in the hypothalamus was relatively lowest.The difference was extremely significant(p<0.01).The expression levels of CYP19 A1 mRNA in the five gonad tissues examined in the Qianbei Ma goats are as follows:ovary>uterus>oviduct>pituitary>hypothalamus;the expression levels of STS mRNA in the five gonad tissues examined as follows:ovary>pituitary>uterus>oviduct>hypothalamus.The r esults provide a basis for further research on the genetic mechanism of CYP19 A1 and STS genes in goats.