咖啡因对胎鼠和成年小鼠海马神经元细胞增殖的影响

Effects of caffeine on the proliferation of hippocampal neurons in fetal and adult mice

目的研究咖啡因对胎鼠和成年小鼠神经细胞增殖能力的影响。方法孕鼠和非孕成年小鼠随机分组,高、低剂量组每天分别给予30、6 mg/kg剂量的咖啡因进行灌胃14 d。分离孕15 d的胎鼠及成年小鼠的海马神经元细胞进行原代培养,绘制细胞生长曲线及MTT法检测各组细胞增殖情况。结果胎鼠来源的神经元细胞,低剂量咖啡因无显著的影响,而高剂量组生长曲线明显高于对照组,MTT试验也显示该组细胞存活率(122.1±6.9)%高于对照组和低剂量组;成年小鼠的神经细胞试验中,低剂量组细胞生长曲线高抬,高剂量组生长曲线则低于对照组,MTT试验也证实了低剂量组细胞存活率(117.1±6.2)%最高,高剂量组(84.7±5.0)%最低。结论对于胎鼠,孕母鼠摄入较高剂量咖啡因不会影响其神经细胞增殖,但是存在其他妊娠危险。对非孕成年鼠,低剂量咖啡因不影响神经细胞,高剂量则将降低活细胞率。因此建议孕期避免摄入咖啡因,非孕成年人少量摄入咖啡因并无坏处,但是不宜过量摄入。

Objective To study the effect of caffeine on proliferation of nerve cell in fetal and adult mice. Methods The pregnant mice and non-pregnant adult mice were randomly assigned. The high-dose or low-dose groups were given 30 or 60 mg/kg of caffeine each day for 14 days. Hippocampal neuron cells from 15 d fetal mice and adult mice were isolated for primary culture,cell proliferation in each group was detected by the plotted cell growth curve and MTT. Results The low dose of caffeine had no significant effect on fetal rat neurons,while the growth curve of the high-dose group was significantly higher than that of the control group. The MTT test also showed that the cell survival rate of the high-dose group( 122. 1±6. 9) % was higher than that of the control group and the low-dose group. In the nerve cell experiment of adult mice,the growth curve of the low-dose group was higher,while that of the high-dose group was lower than that of the control group. By MTT experiment,it was also confirmed that the survival rate of the low-dose group was the highest( 117. 1±6. 2) %,and that of the high-dose group was the lowest( 84. 7± 5. 0) %. Conclusion Higher caffeine intake in pregnant mice did not affect nerve cell proliferation in fetal mice,but there were other pregnancy risks. In non-pregnant adult mice,low doses of caffeine did not affect nerve cells,while high doses reduced the rate of living cells. Therefore,it is recommended to avoid caffeine during pregnancy. It is not harmful for nonpregnant adults to consume small amounts of caffeine,but excessive use should be avoided.