摘要
CRISPR/Cas9 is an adaptive immunity system in bacteria and most archaea(Koonin and Makarova,2009;Horvath and Barrangou,2010).The CRISPR/Cas9 gene editing system is comprised of two key components,a small guide RNA(gRNA)and a Cas9 endonuclease(Deltcheva etal.,2011;Jineketal.,2012).
基金
This work was supported by the Lundbeck Foundation(R219-2016-1375 and R173-2014-1105)
the Danish Research Council for Independent Research(DFF-1337-00128 and 9041-00317B)
the Sapere Aude Young Research Talent Prize(DFF-1335-00763A)
the Innovation Fund Denmark(BrainStem)
Aarhus University Strategic Grant(AU-iCRISPR)
the Sanming Project of Medicine in Shenzhen(SZ5M201612074)
BGIResearch,and Guangdong Provincial Key Laboratory of Genome Read and Write(2017B030301011)。
