全反式维甲酸调节GAS5基因表达诱导人胃癌细胞凋亡

All-trans-retinoic Acid Controls GAS5 Gene Expression and Induces Gastric Cancer Cells Death

在前期研究中我们发现用全反式维甲酸(all-trans-retinoic acid,ATRA)诱导胚胎干细胞分化时长链非编码RNA GAS5的表达呈下调状态。基于癌细胞和胚胎干细胞无限增殖的共性,本研究拟探究ATRA对人胃癌细胞生长的调节作用及其对长链非编码RNA GAS5表达量的影响。研究首先用CCK-8试剂盒和qPCR检测不同浓度ATRA对人胃癌细胞SGC-7901的增殖及GAS5基因表达的影响。结果显示:ATRA在诱导胃癌细胞死亡的同时可上调GAS5基因的表达。进一步的流式细胞检测结果显示过表达GAS5即可诱导胃癌细胞凋亡。因此,ATRA可通过上调GAS5基因的表达诱导胃癌细胞凋亡。

In previous research,we found that embryonic stem cells treated with all-trans-retinoic acid(ATRA)for differentiation have a lower expression level of long non-coding RNA GAS5 than that of control cells.Based on the same characteristic,self-renewal,exist in cancer and embryonic stem cells,here,we further explored whether ATRA regulates human gastric cancer cells’ proliferation and GAS5 expression.Gastric cancer cells SGC-7901 were firstly treated with different concentration of ATRA,then,CCK-8 kits and qPCR methods were used to detect the cells proliferation and GAS5 changes.The results showed that ATRA induced gastric cancer cells apoptosis and increased GAS5 expression.Further examination for GAS5 over-expressed SGC-7901 cells proliferation using flow cytometry showed that over-expressing of GAS5 promoted SGC-7901 cells death.So,it’s possible that ATRA promote gastric cancer cells apoptosis by increasing GAS5 gene expression.