云南美人蕉病毒病病原鉴定

Identification of the Canna indica viral disease causing pathogens in Yunnan Pro-vince

美人蕉(Canna indica)属美人蕉科(Cannaceae)美人蕉属(Canna)多年生宿根草本植物,原产美洲、印度、马来半岛等热带地区[1],目前在热带、亚热带和温带地区均有种植和分布,在我国南方大部分省区常用于园林花卉。美人蕉感染病毒后主要表现为花叶、坏死、褪绿、条纹、植株矮化等症状,影响美人蕉的正常生长及观赏价值。

In this study, the causal agents were identified from Canna indica viral diseased plants in Yunnan Province. The diseased C. indica plants mainly exhibited the symptoms like veinal chlorosis and yellowing, streak mosaic or interveinal chlorosis, while older leaves always showed veinal necrosis as well as chlorosis. Viral pathogens were detected by RT-PCR/PCR in 24 diseased C. indica samples collected from Kunming and Yuxi City in Yunnan Province. The results indicated that the main C. indica-infecting viruses were canna yellow mottle virus(CaYMV), bean yellow mosaic virus(BYMV), sugarcane mosaic virus(SCMV). CaYMV showed the highest detection rate of 87.5 %, whereas, the BYMV had the lowest rate of 16.7% in the 24 samples. Co-infections of CaYMV+SCMV, CaYMV+BYMV and CaYMV+SCMV+BYMV were also detected in the diseased samples. However, cucumber mosaic virus(CMV), tobamovirus, luteovirus, orthotospovirus, begomovirus and umbravirus were not detected in these samples. This is the first report of CaYMV and SCMV infecting C. indica in Yunnan province.