白蚁菌圃中产纤维素酶菌株的鉴定与酶活研究

Identification of Strain with Cellulase Activity in Termite Combsare and Study on Enzymatic Activity

[目的]提高生物质秸秆降解速度,能够更加充分地利用微生物资源。[方法]以黑翅土白蚁菌圃为菌源,采用平板稀释法初筛分离得到降解纤维素的目标菌株,再复筛得到最优菌株FUN-ce4,然后对其进行ITS序列分析鉴定以及酶稳定性研究。[结果]菌株FUN-4归属于栓孔菌属(Trametes)。经对酶活性研究,菌株FUN-4在温度为60℃、pH为4、发酵时间为6 d时酶活相对较高;而温度为40℃、最适pH为5时酶活相对较稳定。[结论]白蚁菌圃中存在活性较高的纤维素降解菌株,可以成为高活性木质纤维素降解酶的重要来源。

[Objective]To improve the degradation rate of biomass straw and make full use of microbial resources.[Method]The target strains degrading cellulose from termite combsare was isolated by plate dilution method,and then the optimal strain FN-4 was rescreened from the target strains,and ITS sequence analysis was conducted combining with the research of the enzymatic activity.[Result]The strain FN-4 was identified as Trametes.The enzymatic activity research experiment showed that the enzymatic activity of strain FUN-4 was relatively high when the temperature was 60℃,the pH was 4,and the fermentation time was 6 days.When the temperature was 40℃ and the pH was 5,the enzyme activity was relatively stable.[Conclusion]There are high activity cellulose degrading strains in termites fungus gardens,which can be an important source of high activity lignocellulosic degrading enzymes.